Cancer Biology

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    Protocols in Current Issue
    In situ Hybridization (ISH) and Quantum Dots (QD) of miRNAs
    Authors:  Sajni Josson, Murali Gururajan and Leland W.K. Chung, date: 02/20/2017, view: 5966, Q&A: 0
    [Abstract] miRNA are short non-coding RNA which inhibit translation of mRNA. miRNA regulate several cellular processes. Certain miRNA are known to induce oncogenesis. miRNA can be measured by real-time PCR and be imaged using a combination of in situ hybridization (ISH) and quantum dots (QD). The advantage of using quantum dots is that several miRNA ...
    miRNA Characterization from the Extracellular Vesicles
    Authors:  Sajni Josson, Murali Gururajan and Leland W.K. Chung, date: 02/20/2017, view: 4124, Q&A: 0
    [Abstract] Cancer cells and cancer associated stromal cells co-evolve secrete extracellular vesicles to the surrounding regions and regulate several processes involved in cancer metastasis. miRNAs have been known to be mediators of cancer progression and metastasis. miRNAs consist of short noncoding RNA. miRNAs are stable in extracellular fluids such as ...
    Polysome Fractionation to Analyze mRNA Distribution Profiles
    Authors:  Amaresh C. Panda, Jennifer L. Martindale and Myriam Gorospe, date: 02/05/2017, view: 13775, Q&A: 4
    [Abstract] Eukaryotic cells adapt to changes in external or internal signals by precisely modulating the expression of specific gene products. The expression of protein-coding genes is controlled at the transcriptional and post-transcriptional levels. Among the latter steps, the regulation of translation is particularly important in cellular processes that ...
    Affinity Pulldown of Biotinylated RNA for Detection of Protein-RNA Complexes
    Authors:  Amaresh C Panda, Jennifer L. Martindale and Myriam Gorospe, date: 12/20/2016, view: 13517, Q&A: 0
    [Abstract] RNA-binding proteins (RBPs) have recently emerged as crucial players in the regulation of gene expression. The interactions of RBPs with target mRNAs control the levels of gene products by altering different regulatory steps, including pre-mRNA splicing and maturation, nuclear mRNA export, and mRNA stability and translation (Glisovic et al ...



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