Cancer Biology

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    Protocols in Current Issue
    In vivo Tumor Growth and Spontaneous Metastasis Assays Using A549 Lung Cancer Cells
    [Abstract] Metastasis accounts for the majority of cancer related deaths. The genetically engineered mouse (GEM) models and cell line-based subcutaneous and orthotopic mouse xenografts have been developed to study the metastatic process. By using lung cancer cell line A549 as an example, we present a modified protocol to establish the cell line-based ...
    Protocol for Murine/Mouse Platelets Isolation and Their Reintroduction in vivo
    Authors:  Jae Hong Im and Ruth J. Muschel, date: 02/20/2017, view: 12832, Q&A: 1
    [Abstract] Platelets and coagulation have long been known to be essential for metastasis in experimental models. In order to study the interactions between tumor cells, platelets and endothelium, we have adapted methods used in coagulation research for the isolation of platelets and their reintroduction into mice. Anti-coagulated murine blood served as the ...
    Immunostaining Protocol: P-Smad2 (Xenograft and Mice)
    [Abstract] Metastasis depends on a gene program expressed by the tumor microenvironment upon TGF-beta stimulation. CRC (Colorectal cancer) cell lines did not induce robust stromal TGF-beta responses when injected into nude mice as shown by lack of p-SMAD2 accumulation in tumor-associated stromal cells. To enforce high TGF-beta signaling in xenografts, we ...



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