Cancer Biology

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    Protocols in Current Issue
    PhagoKinetic Track Assay: Imaging and Analysis of Single Cell Migration
    [Abstract] Cell migration is a highly complex and dynamic biological process, essential in several physiological phenomena and pathologies including cancer dissemination and metastasis formation. Thus understanding single cell migration is highly relevant and requires a suitable image-based assay. Depending on the speed of the moving cells, one may require ...
    Invadopodia Detection and Gelatin Degradation Assay
    Author:  Begoña Díaz, date: 12/20/2013, view: 16170, Q&A: 0
    [Abstract] This protocol is designed to quantify invadopodia formation and activity. Invadopodia are protrusive structures elaborated by cancer cells that mediate cell attachment and remodeling of the extracellular matrix. These structures contribute to the ability of cancer cells to invade and metastasize. In this protocol, both the presence of invadopodia ...
    Transwell Cell Migration Assay Using Human Breast Epithelial Cancer Cell
    Author:  Yanling Chen, date: 02/20/2012, view: 60335, Q&A: 6
    [Abstract] Transwell migration assays have been widely used for studying the motility of different types of cells including metastatic cancer cells. The assay is also useful in screens for compounds that act as chemoattractants or inhibitors of chemotaxis for cells. The assay employs a permeable layer of support, usually a tissue-culture-treated microporous ...



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