In vivo Tumor Growth and Spontaneous Metastasis Assays Using A549 Lung Cancer Cells
[Abstract] Metastasis accounts for the majority of cancer related deaths. The genetically engineered mouse (GEM) models and cell line-based subcutaneous and orthotopic mouse xenografts have been developed to study the metastatic process. By using lung cancer cell line A549 as an example, we present a modified protocol to establish the cell line-based ...
Isolation of Mammary Epithelial Cells and Fibroblasts From Mouse Tumor
Author: Shiva Kazerounian,
date: 02/20/2014,
view: 16888,
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[Abstract] Developing cancer therapeutics requires the ability to investigate their effects using in vitro models of a specific type of tumor. This protocol provides a method for the isolation and adoption to growth in culture of cells from primary tumors. This is particularly valuable for studying mouse models where original tumor cells can be ...