Protocols in Current Issue
    Random DNA Binding Selection Assay (RDSA)
    Authors:  Yafei Wang and Meizhong Luo, date: 04/20/2015, view: 3003, Q&A: 0
    [Abstract] Protein-DNA interaction is a very important cellular process, by which regulation of DNA biological function, usually gene expression, is exerted. The method of random DNA binding selection assay (RDSA) can be used to identify DNA elements bound by proteins with DNA-binding activities. This method is based on the enrichment of the target DNA ...
    Determination of Protein-DNA (ZMYND11-DNA) Interaction by a Label-Free Biolayer Interferometry Assay
    Authors:  Yuan-Yuan Li, Hong Wen, Xiao-Bing Shi and Hai-Tao Li, date: 02/20/2015, view: 5174, Q&A: 0
    [Abstract] This protocol describes a robust technique for the measurement of ZMYND11-DNA interaction by a label-free Biolayer Interferometry (BLI). ZMYND11 is a novel histone reader protein that specifically recognizes H3.3K36me3 via its tandem Bromodomain, zinc-finger and PWWP domain (BP). ZMYND11 links the histone-variant-mediated transcription elongation ...