Protocols in Current Issue
    Testing for Assortative Mating by Diet in Drosophila melanogaster
    Authors:  Philip T Leftwich and Tracey Chapman, date: 10/20/2018, view: 1019, Q&A: 0
    [Abstract] Experimental studies of the evolution of reproductive isolation in real time are a powerful way to reveal the way that fundamental processes, such as mate choice, initiate divergence. Mate choice, while frequently described in females, can occur in either sex, and can be affected by the genetics or environment of an individual. Here we describe ...
    Drosophila Endurance Training and Assessment of Its Effects on Systemic Adaptations
    Authors:  Deena Damschroder , Tyler Cobb, Alyson Sujkowski and Robert Wessells, date: 10/05/2018, view: 1250, Q&A: 0
    [Abstract] Exercise induces beneficial systemic adaptations that reduce the incidence of age-related diseases. However, the molecular pathways that elicit these adaptations are not well understood. Understanding the molecular mechanisms that underlie the exercise response can lead to widely beneficial therapies. Large populations, relatively short lifespan, ...
    Flight and Climbing Assay for Assessing Motor Functions in Drosophila
    Authors:  Steffy B Manjila and Gaiti Hasan, date: 03/05/2018, view: 3187, Q&A: 0
    [Abstract] Motor control requires the central nervous system to integrate different sensory inputs and convey this information to the relevant central pattern generator for execution of motor function through motor neurons and muscles. Proper motor control is essential for any mobile organism to survive and interact with the external environment. For flying ...
    Proboscis Extension Reflex in Apis mellifera [Honeybee] with Only One Antenna
    Authors:  Yu Guo, Zilong Wang, Zhijiang Zeng, Shaowu Zhang and Runsheng Chen, date: 12/05/2017, view: 2689, Q&A: 0
    [Abstract] The proboscis extension reflex (PER) is a common classical conditioned reflex which is widely used in the neurology and ethology. In honeybees, PER experiments can train bees to associate an odor with a reward or punishment. Here we present a variation of the PER experiment in Apis mellifera that trains honeybees using only one antenna. ...
    Aldicarb-induced Paralysis Assay to Determine Defects in Synaptic Transmission in Caenorhabditis elegans
    Authors:  Kelly H. Oh and Hongkyun Kim, date: 07/20/2017, view: 3398, Q&A: 0
    [Abstract] Aldicarb treatment causes an accumulation of acetylcholine in the synaptic cleft of the neuromuscular junction, resulting in sustained muscle activation and eventually paralysis. Aldicarb-induced paralysis assay is an easy and fast method to determine whether synaptic transmission of a C. elegans mutant of interest is altered. This assay ...
    Behavioral and Functional Assays for Investigating Mechanisms of Noxious Cold Detection and Multimodal Sensory Processing in Drosophila Larvae
    Authors:  Atit A. Patel and Daniel N. Cox, date: 07/05/2017, view: 4163, Q&A: 0
    [Abstract] To investigate cellular, molecular and behavioral mechanisms of noxious cold detection, we developed cold plate behavioral assays and quantitative means for evaluating the predominant noxious cold-evoked contraction behavior. To characterize neural activity in response to noxious cold, we implemented a GCaMP6-based calcium imaging assay enabling ...
    Oxidative Stress Assays (arsenite and tBHP) in Caenorhabditis elegans
    Authors:  Collin Yvès Ewald, John M. Hourihan and T. Keith Blackwell, date: 07/05/2017, view: 5485, Q&A: 0
    [Abstract] Cells and organisms face constant exposure to reactive oxygen species (ROS), either from the environment or as a by-product from internal metabolic processes. To prevent cellular damage from ROS, cells have evolved detoxification mechanisms. The activation of these detoxification mechanisms and their downstream responses represent an overlapping ...
    Electroshock Induced Seizures in Adult C. elegans
    Authors:  Monica G Risley, Stephanie P Kelly and Ken Dawson-Scully, date: 05/05/2017, view: 3882, Q&A: 0
    [Abstract] The nematode Caenorhabditis elegans is a useful model organism for dissecting molecular mechanisms of neurological diseases. While hermaphrodite C. elegans contains only 302 neurons, the conserved homologous neurotransmitters, simpler neuronal circuitry, and fully mapped connectome make it an appealing model system for ...
    Artificial Optogenetic TRN Stimulation of C. elegans
    Authors:  Ithai Rabinowitch, Millet Treinin and Jihong Bai, date: 10/20/2016, view: 4265, Q&A: 0
    [Abstract] Optogenetics is a powerful tool for manipulating neuronal activity with high temporal and spatial precision. In the nematode C. elegans optogentics is especially useful and easy to apply. This is because C. elegans is translucent, so its neurons are highly accessible to optic stimulation. In addition, many of its neurons can be ...
    Evaluation of Burkholderia cepacia Complex Bacteria Pathogenicity Using Caenorhabditis elegans
    Authors:  Pietro Tedesco, Elia Di Schiavi, Fortunato Palma Esposito and Donatella de Pascale, date: 10/20/2016, view: 3459, Q&A: 0
    [Abstract] This protocol describes two biological assays to evaluate pathogenicity of Burkholderia cepacia complex (Bcc) strains against the nematode Caenorhabditis elegans. Specifically, these two assays allow one to identify if the under-investigated Bcc strains are able to kill the nematodes by intestinal colonization (slow killing ...

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