Microbiology

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    Protocols in Current Issue
    CytoTrap Two-Hybrid Screening Assay
    Authors:  Lei Zhang, Liqun Du and B. W. Poovaiah, date: 12/05/2014, view: 9530, Q&A: 0
    [Abstract] CytoTrap two-hybrid system provides an alternate strategy to detect protein-protein interactions in yeast. In this system, bait protein is fused with human son of sevenless (hSos) protein (Li et al., 1993), and a cDNA library or prey protein is expressed by fusion with myristoylation signal which anchors the prey fusion protein to yeast ...



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