Neuroscience

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    Protocols in Current Issue
    Precise Targeting of Single Microelectrodes to Orientation Pinwheel Centers
    Authors:  Xue Mei Song, Ming Li, Tao Xu, Dewen Hu and Anna Wang Roe, date: 06/05/2020, view: 359, Q&A: 0
    [Abstract] In the mammalian visual system, early stages of visual form perception begin with orientation selective neurons in primary visual cortex (V1). In many species (including humans, monkeys, tree shrews, cats, and ferrets), these neurons are organized in pinwheel-like orientation columns. To study the functional organization within orientation ...
    Minimally Invasive Oral Surgery Induction of the FRICT-ION Chronic Neuropathic Pain Model
    Authors:  Marena A. Montera and Karin N. Westlund, date: 04/20/2020, view: 590, Q&A: 0
    [Abstract] An easily induced preclinical trigeminal neuropathic nerve injury model is described here for the study of chronic pain, the model acronym FRICT-ION (Foramen Rotundum Inflammatory Constriction Trigeminal InfraOrbital Nerve). In patients, neuropathic pain is thought to be related to ...
    A Simple and Fast Battery Test for Phenotypic Characterization of Mice
    [Abstract] Despite the great number of test batteries already known to assess the behavior of genetically modified and inbred strains of mice, only a few of them focus on basic neurological parameters. The purpose of the battery test proposed is to settle a specific methodology to characterize the phenotype of neurological disease models in mutant or ...
    Semi-automated Model to Accurately Counting Sympathetic Nervous Fibers
    [Abstract] In recent years, the role of sympathetic nervous fibers in chronic inflammation has become increasingly evident. At the onset of inflammation, sympathetic activity is increased in the affected tissue. However, sympathetic fibers are largely absent from chronically inflamed tissues. Apparently, there is a very dynamic relationship between ...
    Optogenetic Food Odor Avoidance Assay
    Authors:  Jay M. Patel, Jessica Swanson and Benjamin R. Arenkiel, date: 10/20/2019, view: 966, Q&A: 0
    [Abstract] Appetite is tightly linked to the sensory experience of feeding, including the smell, taste, and sight of food. Sensory perception can affect the palatability of food, modulating appetite beyond homeostatic requirements. Hypothalamic neurons that govern feeding are responsive to sensory cues associated with food, including food odors. However, the ...
    Cylinder Test to Assess Sensory-motor Function in a Mouse Model of Parkinson’s Disease
    [Abstract] Parkinson’s disease is a progressive neurodegenerative movement disorder that happens due to the loss of dopaminergic neurons in the substantia nigra. The deficiency of dopamine in the basal nuclei drives cardinal motor symptoms such as bradykinesia and hypokinesia. The current protocol describes the cylinder test, which is a relatively simple ...
    Whisker Nuisance Test: A Valuable Tool to Assess Tactile Hypersensitivity in Mice
    Authors:  Luigi Balasco, Gabriele Chelini, Yuri Bozzi and Giovanni Provenzano, date: 08/20/2019, view: 1504, Q&A: 0
    [Abstract] Abnormal response to tactile stimulation, described as both hyper- and hypo-reactivity, is a common sensory impairment in multiple neuropsychiatric disorders. The neural bases of tactile sensitivity remain so far unknown. In the last years, animal studies have proven to be useful for shedding light on the cellular and molecular mechanism ...
    A High-throughput qPCR-based Method to Genotype the SOD1G93A Mouse Model for Relative Copy Number
    Authors:  Valerie R. Tassinari and Fernando G. Vieira, date: 06/20/2019, view: 2099, Q&A: 0
    [Abstract] The most commonly used mouse model in ALS preclinical research expresses multiple copies of the human SOD1 (G93A) transgene. During the course of breeding, successive generations of mice can lose copies of the transgene. Because shorter lifespan of these mice is dependent on transgene copy number, it is essential to ensure that no low-copy, and ...
    Accessing Olfactory Habituation in Drosophila melanogaster with a T-maze Paradigm
    Authors:  Ourania Semelidou, Summer F. Acevedo and Efthimios M.C. Skoulakis, date: 06/05/2019, view: 2192, Q&A: 0
    [Abstract] Habituation is the process whereby perceptual changes alter the value of environmental stimuli, enabling salience filtering. This behavioral response decrement is a form of non-associative learning, where the subject learns about the stimulus and does not involve sensory adaptation, sensory or motor fatigue. The range of behavioral responses in D. ...
    Assessing Olfaction Using Ultrasonic Vocalization Recordings in Mouse Pups with a Sono-olfactometer
    Authors:  Sébastien Wagner, Pierre-Marie Lledo and Françoise Lazarini, date: 02/20/2019, view: 2516, Q&A: 0
    [Abstract] Olfaction is the first sensory modality to develop during fetal life in mammals, and plays a key role in the various behaviors of neonates such as feeding and social interaction. Odorant cues (i.e., mother or predator scents) can trigger potentiation or inhibition of ultrasonic vocalizations (USV) emitted by pups following their ...



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