A Mouse Model of Postoperative Pain
Authors: Ashley M. Cowie and
Cheryl L. Stucky,
date: 01/20/2019,
view: 1312,
Q&A: 0
[Abstract] Postoperative pain is highly debilitating and hinders recovery. Opioids are the main pain medication used for acute postoperative pain. Given the devastating opioid addiction and overdose epidemic across the US, non-opioid pain therapeutics are desperately needed. In order to develop novel, non-opioid therapies for the treatment of postoperative ...
Artificial Inhalation Protocol in Adult Mice
Authors: Thomas P. Eiting and
Matt Wachowiak,
date: 09/20/2018,
view: 1054,
Q&A: 0
[Abstract] Research in the area of in vivo olfactory physiology benefits from having direct access to the nasal airways through which odorants can be presented. Ordinarily, the passage of odorants through the airways is controlled by respiratory rhythm. This fact makes it difficult to control the timing and strength of an olfactory stimulus, since ...
A Fluorescent Dye Method Suitable for Visualization of One or More Rat Whiskers
[Abstract] Visualization and tracking of the facial whiskers is critical to many studies of rodent behavior. High-speed videography is the most robust methodology for characterizing whisker kinematics, but whisker visualization is challenging due to the low contrast of the whisker against its background. Recently, we showed that fluorescent dye(s) can be ...
Assaying Thermo-nociceptive Behavior in Drosophila Larvae
[Abstract] Thermo-nociception, the detection and behavioral response to noxious temperatures, is a highly conserved action to avoid injury and ensure survival. Basic molecular mechanisms of thermal responses have been elucidated in several model organisms and are of clinical relevance as thermal hypersensitivity (thermos-allodynia) is common in neuropathic ...
Assaying Mechanonociceptive Behavior in Drosophila Larvae
[Abstract] Drosophila melanogaster larvae have been extensively used as a model to study the molecular and cellular basis of nociception. The larval nociceptors, class IV dendritic arborization (C4da) neurons, line the body wall of the animal and respond to various stimuli including noxious heat and touch. Activation of C4da neurons results in a ...
Behavioral Assays to Study Oxygen and Carbon Dioxide Sensing in Caenorhabditis elegans
[Abstract] Animals use behavioral strategies to seek optimal environments. Population behavioral assays provide a robust means to determine the effect of genetic perturbations on the ability of animals to sense and respond to changes in the environment. Here, we describe a C. elegans population behavioral assay used to measure locomotory responses ...
Formalin Murine Model of Pain
[Abstract] Pain research is mostly based on experimental assays that use animal models, which may allow deciphering the physiopathology of this condition and to propel drug discovery. The formalin nociception test is considered one of the most predictive approaches to study acute pain in rodents. This test permits monitoring pain-related responses (i.e. ...
Proboscis Extension Reflex in Apis mellifera [Honeybee] with Only One Antenna
[Abstract] The proboscis extension reflex (PER) is a common classical conditioned reflex which is widely used in the neurology and ethology. In honeybees, PER experiments can train bees to associate an odor with a reward or punishment. Here we present a variation of the PER experiment in Apis mellifera that trains honeybees using only one antenna. ...
Assessment of Aversion of Acute Pain Stimulus through Conditioned Place Aversion
[Abstract] Pain is a complex experience. The aversive component of pain has been assessed through conditioned place aversion in rodents. However, this behavioral test does not allow the evaluation of the aversion of an acute pain stimulus. In Zhang et al. (2017), we provide an updated version of a Conditioned Place Aversion paradigm to address this ...