Charging State Analysis of Transfer RNA from an α-proteobacterium
Authors: Liang Yin and
Caroline S. Harwood,
date: 12/05/2020,
view: 501,
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[Abstract] Transfer RNA (tRNA) is an essential link between the genetic code and proteins. During the process of translation, tRNA is charged with its cognate amino acid and delivers it to the ribosome, thus serving as a substrate of protein synthesis. To analyze the charging state of a particular tRNA, total RNA is purified and analyzed on an acid-urea gel. ...