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Generation of Giant Unilamellar Vesicles (GUVs) Using Polyacrylamide Gels

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Charging State Analysis of Transfer RNA from an α-proteobacterium

Authors: Liang Yin and Caroline S. Harwood, date: 12/05/2020, view: 501, Q&A: 0

[Abstract] Transfer RNA (tRNA) is an essential link between the genetic code and proteins. During the process of translation, tRNA is charged with its cognate amino acid and delivers it to the ribosome, thus serving as a substrate of protein synthesis. To analyze the charging state of a particular tRNA, total RNA is purified and analyzed on an acid-urea gel. ...

Ribosome Purification from an α-proteobacterium and rRNA Analysis by Northern Blot

Authors: Liang Yin and Caroline S. Harwood, date: 12/05/2020, view: 483, Q&A: 0

[Abstract] Ribosomes are an integral part of cellular life. They are complex molecular machines consisting of multiple ribosomal proteins and RNAs. To study different aspects of ribosome composition, many methods have been developed over the decades. Here, we describe how to purify ribosomes from the α-proteobacterium Rhodopseudomonas palustris ...

Determination of Intracellular Osmolytes in Cyanobacterial Cells

Authors: Xiaoming Tan, Kuo Song, Cuncun Qiao and Xuefeng Lu, date: 04/20/2018, view: 3559, Q&A: 0

[Abstract] Most of the cyanobacteria accumulate osmolytes including sucrose, glucosylglycerol, in their cells in response to salt stress. Here we describe a protocol of our laboratory for extraction and quantification of cyanobacterial intracellular sucrose and glucosylglycerol. We have confirmed this protocol was applicable to at least four kinds of ...

Determination of Survival of Wildtype and Mutant Escherichia coli in Soil

Authors: Yinka Somorin and Conor O'Byrne, date: 07/20/2017, view: 5574, Q&A: 0

[Abstract] E. coli resides in the gastrointestinal tract of humans and other warm-blooded animals but recent studies have shown that E. coli can persist and grow in various external environments including soil. The general stress response regulator, RpoS, helps E. coli overcome various stresses, however its role in soil survival ...

Enriching Acidophilic Fe(II)-oxidizing Bacteria in No-flow, Fed-batch Systems

Authors: Yizhi Sheng, Bradley Kaley and William D. Burgos, date: 02/05/2017, view: 7042, Q&A: 0

[Abstract] Low-pH microbial Fe(II) oxidation occurs naturally in some Fe(II)-rich acid mine drainage (AMD) ecosystems across so-called terraced iron formations. Indigenous acidophilic Fe(II)-oxidizing bacterial communities can be incorporated into both passive and active treatments to remove Fe from the AMD solution. Here, we present a protocol of enriching ...

Calculation of Microorganism Lag Times as a Measure of Adaptative Capability between Different Growth Conditions

Author: Brice Enjalbert, date: 02/05/2016, view: 5026, Q&A: 0

[Abstract] This protocol has been designed as a simple and efficient way to investigate microorganism adaptive capabilities (Enjalbert et al., 2015). It is performed using switch experiments in which cells are initially grown in the first condition (primary cultures), then rapidly switched to the second condition (secondary culture) without ...

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