Biochemistry

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    Protocols in Current Issue
    In vivo Quantification of Alkanes in Escherichia coli
    Authors:  Tabinda Shakeel, Zia Fatma and Syed Shams Yazdani, date: 04/20/2020, view: 1248, Q&A: 0
    [Abstract] Microbial production of alkanes employing synthetic biology tools has gained tremendous attention owing to the high energy density and similarity of alkanes to existing petroleum fuels. One of the most commonly studied pathways includes the production of alkanes by AAR (acyl-ACP (acyl carrier protein) reductase)-ADO (aldehyde deformylating ...
    Methods for Detecting Microbial Methane Production and Consumption by Gas Chromatography
    Authors:  Jared T. Aldridge, Jennie L. Catlett, Megan L. Smith and Nicole R. Buan, date: 04/05/2016, view: 7125, Q&A: 0
    [Abstract] Methane is an energy-dense fuel but is also a greenhouse gas 25 times more detrimental to the environment than CO2. Methane can be produced abiotically by serpentinization, chemically by Sabatier or Fisher-Tropsh chemistry, or biotically by microbes (Berndt et al., 1996; Horita and Berndt, 1999; Dry, 2002; Wolfe, 1982; Thauer, ...
    Extraction and Quantification of Alkanes in Cyanobacteria
    [Abstract] Many species of cyanobacteria accumulate alkanes in their cells. It has been reported that these cyanobacteria accumulate mainly 15 long carbon chain alkane, pentadecane (C15H32), or/and 17 long chain alkane, heptadecane (C17H36). Here we describe a protocol of our laboratory for extraction and ...



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