Plant Science


    Protocols in Current Issue
    An Efficient Inoculation Technique to Assess the Pathogenicity of Pantoea Species Associated to Bacterial Blight of Rice
    Authors:  Kossi Kini, Raoul Agnimonhan, Issa Wonni and Drissa Silue, date: 09/05/2020, view: 1842, Q&A: 0
    [Abstract] Bacteria blight diseases of rice due to several genera of pathogenic bacteria are one of the major constraints worldwide for rice production. The disease can be best managed through host plant resistance sources. For most of these bacteria such as Xanthomonas oryzae pv. oryzae, X. oryzae pv. oryzicola, ...
    Quantification of Thrips Damage Using Ilastik and ImageJ Fiji
    Authors:  Isabella G. S. Visschers, Nicole M. van Dam and Janny L. Peters, date: 04/20/2018, view: 6918, Q&A: 0
    [Abstract] Quantification of insect damage is an essential measurement for identifying resistance in plants. In screening for host plant resistance against thrips, the total damaged leaf area is used as a criterion to determine resistance levels. Here we present an objective novel method for analyzing thrips damage on leaf disc using the freely available ...
    Growth Assay for the Stem Parasitic Plants of the Genus Cuscuta
    Authors:  Volker Hegenauer , Max Welz, Max Körner and Markus Albert, date: 04/20/2017, view: 9389, Q&A: 0
    [Abstract] Cuscuta spp. are widespread obligate holoparasitic plants with a broad host spectrum. Rootless Cuscuta penetrates host stems with so called haustoria to form a direct connection to the host vascular tissue (Dawson et al., 1994; Lanini and Kogan, 2005; Kaiser et al., 2015). This connection allows a steady uptake ...
    Chlorophyll Content Assay to Quantify the Level of Necrosis Induced by Different R Gene/Elicitor Combinations after Transient Expression
    Authors:  C. Jake Harris and David C. Baulcombe, date: 12/05/2015, view: 7715, Q&A: 0
    [Abstract] This assay can be used to rapidly and accurately quantify levels of leaf necrosis induced after transient expression of R genes and elicitor combinations (Harris et al., 2013). It is based on the inverse correlation between level of necrosis and chlorophyll content in leaf tissue. It is adapted from the calculations described by (Strain ...

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