Immunology

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    Protocols in Current Issue
    An Image-based Dynamic High-throughput Analysis of Adherent Cell Migration
    [Abstract]

    In this protocol, we describe a method to monitor cell migration by live-cell imaging of adherent cells. Scratching assay is a common method to investigate cell migration or wound healing capacity. However, achieving homogenous scratching, finding the optimal time window for end-point analysis and performing an objective image analysis imply, even

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    Collagen-induced Arthritis: A Model for Murine Autoimmune Arthritis
    Authors:  Kathryn M. Pietrosimone, Mengyao Jin, Brad Poston and Peng Liu, date: 10/20/2015, view: 11290, Q&A: 0
    [Abstract] Collagen-induced arthritis (CIA) is a common autoimmune animal model used to study rheumatoid arthritis (RA). The development of CIA involves infiltration of macrophages and neutrophils into the joint, as well as T and B cell responses to type II collagen. In murine CIA, genetically susceptible mice (DBA/1J) are immunized with a type II bovine ...



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