Cancer Biology


    Protocols in Current Issue
    A 3D Skin Melanoma Spheroid-Based Model to Assess Tumor-Immune Cell Interactions
    Authors:  Marek Wagner and Shigeo Koyasu, date: 12/05/2020, view: 1423, Q&A: 0
    [Abstract] Three-dimensional (3D) tumor spheroids have the potential to bridge the gap between two-dimensional (2D) monolayer tumor cell cultures and solid tumors with which they share a significant degree of similarity. However, the progression of solid tumors is often influenced by the dynamic and reciprocal interactions between tumor and immune cells. ...
    Adoptive Transfer of Monocytes Sorted from Bone Marrow
    Authors:  Damya Laoui, Eva Van Overmeire, Chloé Abels, Jiri Keirsse and Jo A Van Ginderachter, date: 01/05/2019, view: 5125, Q&A: 0
    [Abstract] Inflammatory Ly6Chi monocytes can give rise to distinct mononuclear myeloid cells in the tumor microenvironment, such as monocytic myeloid-derived suppressor cells (Mo-MDSC), immature macrophages, M2-like tumor-associated macrophages (TAMs), M1-like TAMs or monocyte-derived dendritic cells (Mo-DCs). This protocol describes a method to ...
    In vivo Imaging of Tumor and Immune Cell Interactions in the Lung
    Authors:  Richard N. Hanna, Grzegorz Chodaczek and Catherine C. Hedrick, date: 10/20/2016, view: 7420, Q&A: 0
    [Abstract] Immunotherapy has demonstrated great therapeutic potential by activating the immune system to fight cancer. However, little is known about the specific dynamics of interactions that occur between tumor and immune cells. In this protocol we describe a novel method to visualize the interaction of tumor and immune cells in the lung of live mice, ...
    Macrophage Polarization by Tumor-induced MDSCs Assay
    Authors:  Felipe Vences-Catalán, Minu K. Srivastava and Shoshana Levy, date: 08/20/2016, view: 10346, Q&A: 0
    [Abstract] Myeloid derived suppressor cells (MDSCs) are a subset of granulocytes (immature myeloid cells) that exploit a variety of mechanism to modulate the innate and adaptive immune system. MDSCs are present normally in the body, but their numbers increase during inflammation and in cancer, promoting an immunosuppressive microenvironment. In addition to ...
    Adoptive Transfer of Tumor Expanded Regulatory T Cells (Tregs)
    Authors:  Felipe Vences-Catalán and Shoshana Levy, date: 08/20/2016, view: 8402, Q&A: 0
    [Abstract] Regulatory T cells (Tregs), a subset of CD4+CD25+ T cells, infiltrate tumors and suppress antitumor activity of effector T and NK cells. Depletion of Tregs by anti CD25+ antibodies has been shown to reduce tumor growth and metastasis (Olkhanud et al., 2009). Conversely, adoptive transfer of Tregs induced ...
    In vitro Tumor Cell Migration Assay Using ThinCertsTM (Transwells)
    Author:  Marc A. Schneider, date: 06/05/2016, view: 14755, Q&A: 0
    [Abstract] The high migration rate of tumor cells often results in poor prognosis for the survival of the patients. Here, we describe a protocol to measure the migration of cells using a quantitative assay. The relative tumor cell migration was measured using ThinCertsTM cell culture inserts and a lactate dehydrogenase (LDH) assay to quantify the ...
    Transwell Co-culture of Bone Marrow Macrophages with Tumor Cells
    Authors:  Mackenzie K. Herroon and Izabela Podgorski, date: 05/05/2014, view: 18990, Q&A: 0
    [Abstract] Bone is a primary site of metastasis from prostate and breast cancers. Bone marrow macrophages (BMMs) are mediators of inflammatory processes and are thought to promote tumor growth in the skeletal sites. In order to elucidate how their interactions with tumor cells impact aggressiveness of metastatic tumors in bone in vitro methods are ...

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