Protocols in Current Issue
    Isolation and Long-term Cultivation of Mouse Alveolar Macrophages
    [Abstract] Alveolar macrophages (AM) are tissue-resident macrophages that colonize the lung around birth and can self-maintain long-term in an adult organism without contribution of monocytes. AM are located in the pulmonary alveoli and can be harvested by washing the lungs using the method of bronchoalveolar lavage (BAL). Here, we compared different ...
    Using Stable Isotopes in Bone Marrow Derived Macrophage to Analyze Metabolism
    [Abstract] Using gas chromatography mass spectrometry (GC-MS) to analyze the citric acid cycle (CAC) and related intermediates (such as glutamate, glutamine, GABA, and aspartate) is an analytical approach to identify unexpected correlations between apparently related and unrelated pathways of energy metabolism. Intermediates can be as expressed as their ...
    Phagocytosis Assay of Necroptotic Cells by Cardiac Myofibroblasts
    Authors:  Yuma Horii, Shoichi Matsuda, Kenji Watari, Akiomi Nagasaka, Hitoshi Kurose and Michio Nakaya, date: 09/20/2017, view: 3634, Q&A: 0
    [Abstract] In myocardial infarction (MI), a plenty of cardiomyocytes undergo necrosis and necroptosis due to the lack of oxygen and nutrients. The dead cardiomyocytes are promptly engulfed by phagocytes. When the dead cells are not engulfed, the noxious contents of the cells are released outside, and thus, induce inflammation, and obstruct the function of ...
    An Assay to Determine Phagocytosis of Apoptotic Cells by Cardiac Macrophages and Cardiac Myofibroblasts
    Authors:  Yuma Horii, Shoichi Matsuda, Kenji Watari, Akiomi Nagasaka, Hitoshi Kurose and Michio Nakaya, date: 09/20/2017, view: 4155, Q&A: 0
    [Abstract] In myocardial infarction (MI), a number of cardiomyocytes undergo apoptosis. These apoptotic cardiomyocytes are promptly engulfed by phagocytes. If the dead cells are not engulfed, their noxious contents are released outside, resulting in induction of inflammation. Therefore, the removal of these dead cells is necessary. However, the contribution ...
    Isolation of Lung Infiltrating Cell in Mice
    Authors:  Eduardo P. Amaral, Elena B. Lassunskaia and Maria Regina D’Império-Lima, date: 02/20/2015, view: 12399, Q&A: 0
    [Abstract] Inflammatory lung diseases induce strong leukocyte recruitment into the organ, culminating in pneumonia area formation. Here, we describe the protocol for isolation of lung infiltrating cells. Using this assay, we analyzed the lung cell phenotyping by flow cytometry and spontaneous cytokine production by cultivating lung cells ex vivo ...
    Purification of Tumor-Associated Macrophages (TAM) and Tumor-Associated Dendritic Cells (TADC)
    Authors:  Damya Laoui, Eva Van Overmeire, Jiri Keirsse, Kiavash Movahedi and Jo A Van Ginderachter, date: 11/20/2014, view: 17931, Q&A: 1
    [Abstract] Tumors are heterogeneous microenvironments where complex interactions take place between neoplastic cells and infiltrating inflammatory cells, such as tumor-associated macrophages (TAM) and tumor-associated dendritic cells (TADC). The relevance of tumor-infiltrating mononuclear myeloid cells is underscored by clinical studies showing a correlation ...
    Isolation and in vitro Activation of Mouse Peyer’s Patch Cells from Small Intestine Tissue
    Authors:  Claudia Pastori and Lucia Lopalco, date: 11/05/2014, view: 11604, Q&A: 0
    [Abstract] The lumen of gastrointestinal tract is exposed to several potentially pathogenic microorganisms, thus it is extremely relevant to understand how immunosurveilance can be established. Peyer’s Patches (PPs) are oval or round lymphoid nodules that protrude from the outer wall of the ileum portion of small intestine. PPs contain a high percentage of B ...
    Harvest and Culture of Mouse Peritoneal Macrophages
    Authors:  Mingfang Lu and Alan W. Varley, date: 11/20/2013, view: 26601, Q&A: 1
    [Abstract] Peritoneal macrophages are used as primary macrophages in lots of studies, mainly because they are easy to obtain. Injection of thioglycollate broth i.p. induces inflammatory responses and elicits large numbers of macrophages. This protocol can be used for harvesting resident or thioglycollate-elicited peritoneal cells. Peritoneal macrophages are ...
    Determination of Toxoplasma gondii Replication in Naïve and Activated Macrophages
    Authors:  Emma Iaconetti, Brian Lynch, Nathaniel Kim and Dana G. Mordue, date: 11/20/2012, view: 9194, Q&A: 0
    [Abstract] Toxoplasma gondii is an obligate intracellular protozoan parasite that causes the disease toxoplasmosis. Chronic infection is established through the formation of tissue cysts predominantly in cardiac and neurologic tissues. A defining characteristic of T. gondii is its ability to evade the host’s immune defenses; specifically, ...
    Generation of Mouse Bone Marrow-Derived Macrophages (BM-MFs)
    Authors:  Ivan Zanoni, Renato Ostuni and Francesca Granucci, date: 06/20/2012, view: 13038, Q&A: 1
    [Abstract] Generating mouse macrophages from bone-marrow progenitor cells is a useful tool to study biological functions of mouse macrophages. Macrophages are one of the major populations of phagocytes and play many different roles during inflammatory process initiation and termination.

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