Plant Science

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    Protocols in Current Issue
    Determination of Recombinant Mannitol-1-phosphate Dehydrogenase Activity from Ectocarpus sp.
    Authors:  Agnès Groisillier and Thierry Tonon, date: 11/05/2016, view: 5484, Q&A: 0
    [Abstract] Brown algae belong to a phylogenetic lineage distantly related to green plants and animals, and are found predominantly, but not exclusively, in the intertidal zone, a harsh and frequently changing environment. Because of their unique evolutionary history and of their habitat, brown algae feature several peculiarities in their metabolism. One of ...
    Determination of Recombinant Mannitol-1-phosphatase Activity from Ectocarpus sp.
    Authors:  Agnès Groisillier and Thierry Tonon, date: 08/20/2016, view: 5826, Q&A: 0
    [Abstract] Brown algae belong to a phylogenetic lineage distantly related to green plants and animals, and are found predominantly, but not exclusively, in the intertidal zone, a harsh and frequently changing environment. Because of their unique evolutionary history and of their habitat, brown algae feature several peculiarities in their metabolism. One of ...
    Purification of Rubisco from Chlamydomonas reinhardtii
    [Abstract] Chlamydomonas reinhardtii is a model organism for chloroplast studies. Besides other convenient features, the feasibility of chloroplast genome transformation distinguishes this unicellular alga as ideal for the manipulation of chloroplastic gene expression aiming biotechnological goals, such as improved biofuel and biomass production. ...
    Assay of the Carboxylase Activity of Rubisco from Chlamydomonas reinhardtii
    [Abstract] The performance of the carbon-fixing enzyme, ribulose 1, 5-bisphosphate carboxylase/oxygenase (EC 4.1.1.39, Rubisco), controls biomass accumulation in green plants, algae and most autotrophic bacteria. In particular, the carboxylase activity of Rubisco incorporates carbon from CO2 to ribulose 1, 5-bisphosphate (RuBP) producing two ...
    Pyruvate:ferredoxin Oxidoreductase (PFR1) Activity Assays Using Methyl Viologen as Artificial Electron Acceptor
    Author:  Jens Noth, date: 09/05/2013, view: 9737, Q&A: 0
    [Abstract] Here we describe the activity measurements of heterologous expressed pyruvate:ferredoxin oxidoreductase (Noth et al., 2013) (Noth et al.,2013) from Chlamydomonas reinhardtii. This enzyme catalyzes the reversible reaction (I) from pyruvate to acetyl CoA and CO2 generating low potential electrons which are in ...
    Heterologous Production and Anaerobic Purification of His- and StrepII-tagged Recombinant Proteins
    Author:  Jens Noth, date: 09/05/2013, view: 11839, Q&A: 0
    [Abstract] This protocol describes the heterologous expression and purification of proteins related to anoxic hydrogen production of Chlamydomonas reinhardtii (Noth et al., 2013). For this, the bacterial expression hosts Escherichia coli BL21 (DE3) ΔiscR (Akhtar MK et al., 2008) and Clostridium acetobutylicum ...
    Western Blot Analysis of Chloroplast HSP70B in Chlorella Species
    Authors:  Stephka Chankova, Zhana Mitrovska and Nadezhda Yurina, date: 08/05/2013, view: 7574, Q&A: 0
    [Abstract] Western blotting allows for the specific detection of proteins by an antibody of interest. This protocol utilizes isolation of total proteins protocol for Chlorella vulgaris prior to gel electrophoresis. After electrophoresis, the selected antibodies are used to detect and quantify levels of chloroplast HSP70B.
    Immunoelectron Microscopy in Chlamydomonas Cells
    Authors:  Maria A. Sinetova and Alexandra G. Markelova, date: 03/05/2013, view: 9425, Q&A: 0
    [Abstract] The method of immunoelectron microscopy is intended for localization of proteins inside the cells of Chlamydomonas reinhardtii or other microalgae and cyanobacteria. This protocol was used to study localization of carbonic anhydrase Cah3 with antibodies raised in rabbit, though it can be used to localize any other abundant protein. ...



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