Cell Biology

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    Protocols in Current Issue
    Plaque Assay to Determine Invasion and Intercellular Dissemination of Shigella flexneri in TC7 Human Intestinal Epithelial Cells
    Authors:  Atin Sharma and Andrea Puhar, date: 07/05/2019, view: 629, Q&A: 0
    [Abstract] Shigella flexneri invades the epithelial cells lining the gut lumen and replicates intracellularly. The specialized Type III Secretion System (T3SS) and its effector proteins, encoded on a large virulence plasmid, assist the bacterium to gain access to the cytosol. Thereafter Shigella disseminates to neighboring cells in an ...
    Lymphocyte Isolation, Th17 Cell Differentiation, Activation, and Staining
    Authors:  Pawan Kumar and Jay K Kolls, date: 12/05/2016, view: 7126, Q&A: 1
    [Abstract] In vitro Th17 (α, β T helper cell which produce IL-17A, IL-17F and IL-22) differentiation has been routinely used for functional T cells studies. Here we describe a method for Th17 cell differentiation.
    Pit Assay to Measure the Bone Resorptive Activity of Bone Marrow-derived Osteoclasts
    Authors:  Alexander Vesprey and Wentian Yang, date: 06/20/2016, view: 8059, Q&A: 0
    [Abstract] Although it is possible to use a tartrate-resistant acid phosphatase (TRAP) stain to assist in identifying osteoclasts, a separate method is needed to determine the bone resorption activity of osteoclasts. Since osteoclasts leave “pits” after bone matrix resorption (Charles et al., 2014), it is possible to stain pits as a method of ...
    Fluoro-Jade B Staining for Neuronal Cell Death
    Authors:  Nathalie Laflamme, Paul Préfontaine and Serge Rivest, date: 01/05/2016, view: 13802, Q&A: 2
    [Abstract] Fluoro-Jade is a fluorescent derivative used for histological staining of degenerating neurons. This technique is simple and sensitive enough to label distal dendrites, axons, axon terminals as well as neuronal bodies. Fluoro-Jade has excitation and emission peak of 480 and 525 nanometer respectively. It can be visualized using a fluorescein/FITC ...
    Bone Resorption Assay
    Authors:  Carina Scholtysek, Gerhard Krönke and Georg Schett, date: 07/20/2014, view: 10528, Q&A: 0
    [Abstract] The Bone resorption assay provides an easy to use protocol for quantitatively measuring in vitro osteoclast-mediated bone resorption. Osteoclasts can be seeded onto the bone slices and formation of resorption pits can be quantified via toluidinblue staining (Scholtysek et al., 2013).
    Protoplast Preparation and Determination of Cell Death
    Author:  Antoine Danon, date: 06/20/2014, view: 11480, Q&A: 3
    [Abstract] The protoplasts assay constitutes a powerful tool that allows an easy uptake of active agents and a precise quantification of cell death induction in different populations. Our study showed that the basal level of cell death in our controls is low and stable throughout the length of our experiments (Danon et al., 2005; Pineau et al. ...
    Alkaline Phosphatase Staining
    Author:  Pearl A. Campbell, date: 03/05/2014, view: 20328, Q&A: 0
    [Abstract] Two main features characterize pluripotent cells; self-renewal (unlimited cell division) and the ability to give rise to all cells of the adult organism. Given the recent impact of induced pluripotent stem cells (iPSCs) and ongoing use of pluripotent embryonic stem cells ESCs (ESCs) in basic discovery, drug development, and potential use for stem ...
    Assay to Evaluate Vascular Permeability Induction in Mice
    [Abstract] Dengue virus infection usually courses as a benign self-limited fever, called dengue fever. However, on occasions it can progress to a life-threatening complication known as severe dengue (SD). A hallmark of SD is a sharp increase in vascular permeability. Secondary infections are considered a risk factor to develop SD, presumably through a ...
    Soft Agar Anchorage-independent Assay
    Authors:  Li-Ting Wang and Shih-Hsien Hsu, date: 10/20/2013, view: 10559, Q&A: 2
    [Abstract] Chronic inflammation drives initiation of hepatocellular carcinoma (HCC), but the underlying mechanisms linking inflammation and tumor formation remain obscure. In this study, soft agar anchorage-independent assay were used to determine tumor transform activity of hepatoma cells with ISX over expression or knockdown in vitro.
    Drug Sensitivity Assay of Xanthomonas citri subsp. citri Using REMA Plate Method
    Authors:  Isabel C. Silva and Henrique Ferreira, date: 08/20/2013, view: 7111, Q&A: 0
    [Abstract] Resazurin Microtiter Assay (REMA) is a simple, rapid, reliable, sensitive, safe and cost-effective measurement of cell viability. Resazurin detects cell viability by converting from a nonfluorescent dye to the highly red fluorescent dye resorufin in response to chemical reduction of growth medium resulting from cell growth (Palomino et al. ...



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