Cell Biology

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    Protocols in Current Issue
    Acidified Blue Ink-staining Procedure for the Observation of Fungal Structures Inside Roots of Two Disparate Plant Lineages
    Authors:  Jill Kowal, Elena Arrigoni and Sophie Lane, date: 10/20/2020, view: 218, Q&A: 0
    [Abstract] Identifying microscopic mycorrhizal fungal structures in roots, i.e., hyphae, vesicles and arbuscules, requires root staining procedures that are often time consuming and involves chemicals known to present health risks from exposure. By modifying established protocols, our root staining method stains roots using a safe ink- and ...
    Microscopy-based Methods for Rosetting Assay in Malaria Research
    Authors:  Wenn-Chyau Lee and Laurent Rénia, date: 07/05/2020, view: 1036, Q&A: 0
    [Abstract] In malaria, rosetting phenomenon is a condition where a Plasmodium-infected erythrocyte stably adheres to at least an uninfected erythrocyte. This phenomenon that occurs in all species of human malaria parasite is likely to be an immune escape mechanism for the parasite. However, it has been associated with malaria pathogenesis, possibly ...
    Testing Bone Formation Induction by Calvarial Injection Assay in vivo
    Authors:  Narelle E McGregor, Ingrid J Poulton, Emma C Walker and Natalie A Sims, date: 03/20/2020, view: 1372, Q&A: 0
    [Abstract] Bone formation occurs during embryogenesis, skeletal growth and during the process of skeletal renewal throughout life. In the process of bone formation, osteoblasts lay down a collagen-containing matrix, termed osteoid, which is gradually hardened by incorporation of mineral crystals. Although osteoblasts can be induced to differentiate and to ...
    Plaque Assay to Determine Invasion and Intercellular Dissemination of Shigella flexneri in TC7 Human Intestinal Epithelial Cells
    Authors:  Atin Sharma and Andrea Puhar, date: 07/05/2019, view: 2091, Q&A: 0
    [Abstract] Shigella flexneri invades the epithelial cells lining the gut lumen and replicates intracellularly. The specialized Type III Secretion System (T3SS) and its effector proteins, encoded on a large virulence plasmid, assist the bacterium to gain access to the cytosol. Thereafter Shigella disseminates to neighboring cells in an ...
    Lymphocyte Isolation, Th17 Cell Differentiation, Activation, and Staining
    Authors:  Pawan Kumar and Jay K Kolls, date: 12/05/2016, view: 9413, Q&A: 1
    [Abstract] In vitro Th17 (α, β T helper cell which produce IL-17A, IL-17F and IL-22) differentiation has been routinely used for functional T cells studies. Here we describe a method for Th17 cell differentiation.
    Pit Assay to Measure the Bone Resorptive Activity of Bone Marrow-derived Osteoclasts
    Authors:  Alexander Vesprey and Wentian Yang, date: 06/20/2016, view: 9617, Q&A: 0
    [Abstract] Although it is possible to use a tartrate-resistant acid phosphatase (TRAP) stain to assist in identifying osteoclasts, a separate method is needed to determine the bone resorption activity of osteoclasts. Since osteoclasts leave “pits” after bone matrix resorption (Charles et al., 2014), it is possible to stain pits as a method of ...
    Fluoro-Jade B Staining for Neuronal Cell Death
    Authors:  Nathalie Laflamme, Paul Préfontaine and Serge Rivest, date: 01/05/2016, view: 17229, Q&A: 2
    [Abstract] Fluoro-Jade is a fluorescent derivative used for histological staining of degenerating neurons. This technique is simple and sensitive enough to label distal dendrites, axons, axon terminals as well as neuronal bodies. Fluoro-Jade has excitation and emission peak of 480 and 525 nanometer respectively. It can be visualized using a fluorescein/FITC ...
    Bone Resorption Assay
    Authors:  Carina Scholtysek, Gerhard Krönke and Georg Schett, date: 07/20/2014, view: 12664, Q&A: 0
    [Abstract] The Bone resorption assay provides an easy to use protocol for quantitatively measuring in vitro osteoclast-mediated bone resorption. Osteoclasts can be seeded onto the bone slices and formation of resorption pits can be quantified via toluidinblue staining (Scholtysek et al., 2013).
    Protoplast Preparation and Determination of Cell Death
    Author:  Antoine Danon, date: 06/20/2014, view: 14044, Q&A: 3
    [Abstract] The protoplasts assay constitutes a powerful tool that allows an easy uptake of active agents and a precise quantification of cell death induction in different populations. Our study showed that the basal level of cell death in our controls is low and stable throughout the length of our experiments (Danon et al., 2005; Pineau et al. ...
    Alkaline Phosphatase Staining
    Author:  Pearl A. Campbell, date: 03/05/2014, view: 24288, Q&A: 0
    [Abstract] Two main features characterize pluripotent cells; self-renewal (unlimited cell division) and the ability to give rise to all cells of the adult organism. Given the recent impact of induced pluripotent stem cells (iPSCs) and ongoing use of pluripotent embryonic stem cells ESCs (ESCs) in basic discovery, drug development, and potential use for stem ...



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