A Bioassay Protocol for Quorum Sensing Studies Using Vibrio campbellii
[Abstract] Quorum Sensing (QS), or bacterial cell-to-cell communication, is a finely-tuned mechanism that regulates gene expression on a population density-dependent manner through the production, secretion and reception of extracellular signaling molecules termed autoinducers (AIs). Given that QS plays an important role in bacterial biofilm formation and ...
Western Blotting for Staphylococcus aureus AgrA
Authors: Chikara Kaito and
Kazuhisa Sekimizu,
date: 03/20/2014,
view: 11634,
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[Abstract] Staphylococcus aureus has a quorum sensing system to regulate the expression of various virulence factors, which is exerted by the agr locus that encodes agrBDCA and a regulatory RNA called RNAIII. AgrB, AgrD, and AgrC proteins are involved in producing and recognizing extracellular quorum sensing molecules and transduce ...