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Preparation of Drosophila Polytene Chromosomes, Followed by Immunofluorescence Analysis of Chromatin Structure by Multi-fluorescence Correlations

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Extraction of Small Molecules from Fecal Samples and Testing of Their Activity on Microbial Physiology

Authors: Eduardo S. Alves, Rosana B. R. Ferreira and L. Caetano M. Antunes, date: 04/20/2018, view: 3686, Q&A: 0

[Abstract] The human body is colonized by vast communities of microbes, collectively known as microbiota, or microbiome. Although microbes colonize every surface of our bodies that is exposed to the external environment, the biggest collection of microbes colonizing humans and other mammals can be found in the gastrointestinal tract. Given the fact that the ...

A Flow-assay for Farnesol Removal from Adherent Candida albicans Cultures

Authors: Melanie Polke and Ilse D. Jacobsen, date: 10/05/2017, view: 4522, Q&A: 0

[Abstract] Here, we describe a protocol for a continuous flow system for C. albicans cultures growing adherent to a plastic surface. The protocol was adapted from a previous method established to simulate blood flow on endothelial cells (Wilson and Hube, 2010). The adapted protocol was used by us for the removal of molecules in C. albicans ...

Protein Expression Protocol for an Adenylate Cyclase Anchored by a Vibrio Quorum Sensing Receptor

Authors: Stephanie Beltz and Joachim E. Schultz, date: 01/20/2017, view: 5684, Q&A: 0

[Abstract] The direct regulation of a mycobacterial adenylate cyclase (Rv1625c) via exchange of its membrane anchor by the quorum sensing receptor CqsS (Vibrio harveyi) has recently been reported (Beltz et al., 2016). This protocol describes the expression and membrane preparation for these chimeric proteins.

A Bioassay Protocol for Quorum Sensing Studies Using Vibrio campbellii

Authors: Alberto J. Martín-Rodríguez and José J. Fernández, date: 07/20/2016, view: 7655, Q&A: 0

[Abstract] Quorum Sensing (QS), or bacterial cell-to-cell communication, is a finely-tuned mechanism that regulates gene expression on a population density-dependent manner through the production, secretion and reception of extracellular signaling molecules termed autoinducers (AIs). Given that QS plays an important role in bacterial biofilm formation and ...

Purification and Structural Analysis of QS-inhibiting Compounds from Staphylococcus delphini

Authors: Ya-Yun Chu, Mulugeta Nega and Friedrich Götz, date: 06/05/2014, view: 6139, Q&A: 0

[Abstract] The knowledge that many pathogens rely on cell-to-cell communication mechanisms known as quorum sensing, opens a new disease control strategy: quorum quenching. Here we present a purification protocol for molecules excreted by a group of Gram-positive zoonotic pathogen bacteria, the ‘Staphylococcus intermedius group’, that suppress the ...

Western Blotting for Staphylococcus aureus AgrA

Authors: Chikara Kaito and Kazuhisa Sekimizu, date: 03/20/2014, view: 9615, Q&A: 0

[Abstract] Staphylococcus aureus has a quorum sensing system to regulate the expression of various virulence factors, which is exerted by the agr locus that encodes agrBDCA and a regulatory RNA called RNAIII. AgrB, AgrD, and AgrC proteins are involved in producing and recognizing extracellular quorum sensing molecules and transduce ...

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