Protocols in Current Issue
    Dual-sided Voltage-sensitive Dye Imaging of Leech Ganglia
    Authors:  Yusuke Tomina and Daniel A. Wagenaar, date: 03/05/2018, view: 135, Q&A: 0
    [Abstract] In this protocol, we introduce an effective method for voltage-sensitive dye (VSD) loading and imaging of leech ganglia as used in Tomina and Wagenaar (2017). Dissection and dye loading procedures are the most critical steps toward successful whole-ganglion VSD imaging. The former entails the removal of the sheath that covers neurons in the ...
    Bioluminescence Monitoring of Neuronal Activity in Freely Moving Zebrafish Larvae
    [Abstract] The proof of concept for bioluminescence monitoring of neural activity in zebrafish with the genetically encoded calcium indicator GFP-aequorin has been previously described (Naumann et al., 2010) but challenges remain. First, bioluminescence signals originating from a single muscle fiber can constitute a major pitfall. Second, ...
    Craniotomy for Cortical Voltage-sensitive Dye Imaging in Mice
    Authors:  Takayuki Suzuki and Masanori Murayama, date: 02/05/2016, view: 4833, Q&A: 0
    [Abstract] Cortico-cortical interactions play crucial roles in various brain functions. Here, we present a detailed surgical procedure for cortical voltage-sensitive dye (VSD) imaging that allows monitoring of spatiotemporal dynamics in cortical activity in living mice. Cortical neurons in the upper layers (layer 1-3) are stained with a VSD, and an image ...
    Fluorescent Measurement of Synaptic Activity Using SynaptopHluorin in Isolated Hippocampal Neurons
    Authors:  Hongmei Li, Han-A Park and Elizabeth A. Jonas, date: 12/05/2014, view: 4899, Q&A: 0
    [Abstract] This protocol comprises the entire process of fluorescent measurement of vesicle recycling using the probe SynaptopHluorin, a pH-dependent GFP variant whose fluorescence increases at the synapse upon vesicle release due to fluorescence quenching in acidic vesicles. This technique provides a genetic tool to monitor synaptic vesicle recycling in ...
    Neuronal Morphology Analysis
    Authors:  Désirée R. M. Seib and Ana Martin-Villalba, date: 08/05/2013, view: 5519, Q&A: 0
    [Abstract] This protocol describes how to visualize neuronal morphology and how to determine neuronal complexity of immature and mature hippocampal neurons in the mouse in vivo including tissue preparation, staining of brain sections and confocal cell analysis.
    Hippocampal Neuron Dissociation Transfection and Culture in Microfluidics Chambers
    Author:  Yang Geng, date: 07/20/2012, view: 9295, Q&A: 0
    [Abstract] Microfluidics chamber is an ideal tool to study local events that occurring in neuronal projections by perfectly compartmentalizing the cell soma from certain branches. It is very well suited for live cell imaging or immunohistochemistry staining. This protocol has been carefully modified in detail to fit the requirement of primary rat hippocampal ...