Neuroscience

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    Protocols in Current Issue
    Staining and Quantitative Analysis of Myelinating Oligodendrocytes in the Mouse Grey Matter
    Authors:  Matthew Swire and Charles ffrench-Constant, date: 10/20/2020, view: 225, Q&A: 0
    [Abstract] Oligodendrocytes generate distinct patterns of myelination throughout the CNS. Variations in myelination along axons may enable neurons to fine-tune conduction velocities and alter signal synchronisation. Here we outline a staining protocol permitting the assessment of the number and length of myelin sheaths formed by oligodendrocyte in the mouse ...
    Dissection and Staining of Mouse Brain Ventricular Wall for the Analysis of Ependymal Cell Cilia Organization
    [Abstract] In the developing and mature central nervous system (CNS) the ventricular lumen is lined by the neuroepithelium and ependymal, respectively. These ventricular epithelia perform important functions related to the development, morphogenesis and physiology of the brain. In the mature CNS, ependyma constitutes a barrier between brain parenchyma and ...
    Combined in situ Hybridization/Immunohistochemistry (ISH/IH) on Free-floating Vibratome Tissue Sections
    Author:  Manuel E. Lopez, date: 09/20/2014, view: 11235, Q&A: 0
    [Abstract] In situ hybridization and immunostaining are common techniques for localizing gene expression, the mRNA and protein respectively, within tissues. Both techniques can be applied to tissue sections to achieve similar goals, but in some cases, it is necessary to use them together. For example, complement C1q is a secreted protein complex ...
    Mouse Cochlear Whole Mount Immunofluorescence
    Authors:  Omar Akil and Lawrence R. Lustig, date: 03/05/2013, view: 25708, Q&A: 2
    [Abstract] This protocol comprises the entire process of immunofluorescence staining on mouse cochlea whole mount, starting from tissue preparation to the mounting of the tissue. This technique provides “three-dimensional” views of the stained components in order to determine the localization of a protein of interest in the tissue in its natural state and ...
    Immunofluorescence Staining on Mouse Embryonic Brain Sections
    Author:  Xuecai Ge, date: 06/05/2012, view: 19951, Q&A: 0
    [Abstract] This protocol comprises the entire process of immunofluorescence staining on mouse embryonic brains, starting from tissue preparation to mounting of the tissue sections.



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