Protocols in Current Issue
    Monitoring Natural Killer Cell Function in Human Ovarian Cancer Cells of Ascitic Fluid
    Authors:  Vikash Kumar and Asok Mukhopadhyay, date: 12/20/2018, view: 3728, Q&A: 0
    [Abstract] Natural killer (NK) cells are the major effectors of the innate immune system when activated resulting in modulation of immune response of the host defense through target cell lysis and secretion of cytokines. Precise functions of NK cells are essential for the treatment outcome of different virus infections and malignant diseases. NK cells impart ...
    Measuring Procaspase-8 and -10 Processing upon Apoptosis Induction
    Authors:  Sabine Pietkiewicz, Clara Wolfe, Jörn H. Buchbinder and Inna N. Lavrik, date: 01/05/2017, view: 7531, Q&A: 0
    [Abstract] Apoptosis or programmed cell death is important for multicellular organisms to keep cell homeostasis and for the clearance of mutated or infected cells. Apoptosis can be induced by intrinsic or extrinsic stimuli. The first event in extrinsic apoptosis is the formation of the Death-Inducing Signalling Complex (DISC), where the initiator caspases-8 ...
    Chromium-51 (51Cr) Release Assay to Assess Human T Cells for Functional Avidity and Tumor Cell Recognition
    Authors:  Petra Baumgaertner, Daniel E. Speiser, Pedro Romero, Nathalie Rufer and Michael Hebeisen, date: 08/20/2016, view: 15479, Q&A: 0
    [Abstract] Cytotoxic CD8+ T cells are able to specifically recognize and kill target cells through specific interaction between their T cell receptors (TCRs) and small immunogenic peptides (antigens) presented by major histocompatibility complex (MHC) molecules. The antigen recognition capacity and in vitro lytic activity of ...
    In vivo OVA-specific Cytotoxic CD8+ T Cell Killing Assay
    Authors:  Nada Chaoul, Catherine Fayolle and Claude Leclerc, date: 06/20/2016, view: 15788, Q&A: 0
    [Abstract] Cytotoxic CD8+ T cells are responsible for the lysis of cells expressing peptides associated with MHC class I molecules and derived from infection with a pathogen or from mutated antigens. In order to quantify in vivo this antigen-specific CD8+ T cell killing activity, we use the in vivo killing assay (IVK). ...
    Ex vivo Human Natural Killer (NK) Cell Stimulation and Intracellular IFNγ and CD107a Cytokine Staining
    Authors:  Vanessa A. York and Jeffrey M. Milush, date: 06/20/2015, view: 14380, Q&A: 1
    [Abstract] Natural killer (NK) cells comprise 5–20% of peripheral blood mononuclear cells (PBMC) in humans. In addition to their fundamental roles in the defense against viral infections and tumor surveillance, NK cells help shape adaptive immune responses through their production of cytokines. NK cells are traditionally identified as CD3neg, CD14 ...
    ADCC Assay Protocol
    Authors:  Vikram Srivastava, Zheng Yang, Ivan Fan Ngai Hung, Jianqing Xu, Bojian Zheng and Mei-Yun Zhang, date: 01/20/2014, view: 34826, Q&A: 0
    [Abstract] Antibody-dependent cell-mediated cytotoxicity (ADCC) bridges innate and adaptive immunity, and it involves both humoral and cellular immune responses. ADCC has been found to be a main route of immune protection against viral infections and cancers in vivo. Here we developed a flow cytometry based protocol for ADCC assay using human ...
    Ex vivo Natural Killer Cell Cytotoxicity Assay
    [Abstract] Natural Killer (NK) cells are cytotoxic lymphocytes that constitute a major component of the innate immune system. Immunosurveillance of the host by NK cells for malignant and virally-infected cells results in direct cytotoxicity and the production of cytokines to enhance the immune response. This protocol will describe the “gold standard” ...
    Standard 4-hours Chromium-51 (51Cr) Release Assay
    Author:  Julie Gertner-Dardenne, date: 12/05/2012, view: 20115, Q&A: 0
    [Abstract] 51Cr-release assays are commonly used for the precise and accurate quantification of cytotoxicity, particularly in the study of tumor cytolysis. This test has the advantage of requiring only very few cells.

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