Cell Biology

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    Protocols in Current Issue
    FRET Reporter Assays for cAMP and Calcium in a 96-well Format Using Genetically Encoded Biosensors Expressed in Living Cells
    Authors:  Brandon T. Milliken, Robert P. Doyle, George G. Holz and Oleg G. Chepurny, date: 06/05/2020, view: 839, Q&A: 0
    [Abstract] Stimulation of G protein-coupled receptors (GPCR) by hormones and neurotransmitters elicits cellular responses, many of which result from alterations in the concentrations of cytosolic cAMP and Ca2+. Here, we describe a microplate reader fluorescence resonance energy transfer (FRET) assay that uses the genetically encoded biosensors ...
    Optogenetic Tuning of Ligand Binding to The Human T cell Receptor Using The opto-ligand-TCR System
    [Abstract] T cells are one major cell type of the immune system that use their T cell antigen receptor (TCR) to bind and respond to foreign molecules derived from pathogens. The ligand-TCR interaction half-lives determine stimulation outcome. Until recently, scientists relied on mutating either the TCR or its ligands to investigate how varying TCR-ligand ...
    Endpoint or Kinetic Measurement of Hydrogen Sulfide Production Capacity in Tissue Extracts
    Authors:  Christopher Hine and James R. Mitchell, date: 07/05/2017, view: 6440, Q&A: 0
    [Abstract] Hydrogen sulfide (H2S) gas is produced in cells and tissues via various enzymatic processes. H2S is an important signaling molecule in numerous biological processes, and deficiencies in endogenous H2S production are linked to cardiovascular and other health complications. Quantitation of steady-state H2S ...
    cAMP Accumulation Assays Using the AlphaScreen® Kit (PerkinElmer)
    Authors:  Cassandra Koole, Denise Wootten and Patrick Sexton, date: 07/05/2013, view: 9576, Q&A: 0
    [Abstract] Cyclic adenosine monophosphate (cAMP) is an intracellular signaling messenger derived from the catalytic conversion of ATP, and is a major product of activated Gs protein-coupled receptors. Conversely, formation of cAMP is inhibited by Gi protein-coupled receptors. This protocol has been optimized for the detection of ...



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