Cell Biology

Categories

    Protocols in Current Issue
    Stable Transformation of Arabidopsis thaliana Cell Suspension Cultures: A Case Study for the Overexpression of The COI1 Receptor
    [Abstract] Cell suspension cultures have been studied for decades to produce natural molecules. However, the difficulty in generating stably transformed cell lines has limited their use to produce high value chemicals reproducibly and in elevated quantities.

    In this protocol, a method to stably transform and maintain Arabidopsis cell ...
    In vivo Leukemogenesis Model Using Retrovirus Transduction
    Authors:  Hiroshi Okuda and Akihiko Yokoyama, date: 12/05/2017, view: 6426, Q&A: 0
    [Abstract] Various genetic alterations such as chromosomal translocation cause leukemia. For examples, gene rearrangements of the mixed-lineage leukemia (MLL) gene generate MLL fusion genes, whose products are potent oncogenic drivers in acute leukemia. To better understand the mechanism of disease onset, several murine leukemia models ...
    In vivo Efficacy Studies in Cell Line and Patient-derived Xenograft Mouse Models
    Authors:  Elizabeth A. Tovar, Curt J. Essenburg and Carrie Graveel, date: 01/05/2017, view: 11372, Q&A: 0
    [Abstract] In vivo xenograft models derived from human cancer cells have been a gold standard for evaluating the genetic drivers of cancer and are valuable preclinical models for evaluating the efficacy of cancer therapeutics. Recently, patient-derived tumorgrafts from multiple tumor types have been developed and shown to more accurately ...
    Retrovirus Mediated Malignant Transformation of Mouse Embryonic Fibroblasts
    Authors:  Huei San Leong and Marnie Blewitt, date: 08/05/2013, view: 9420, Q&A: 2
    [Abstract] Cellular transformation is a widely used method to artificially induce cells to form tumours in vivo. Here, we describe the methodology for malignant transformation of mouse embryonic fibroblasts (MEFs) for transplantation into immunodeficient nude mice, as used in Leong et al. (2013). The two-step process involves: 1) ...



    We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.