Molecular Biology

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    Protocols in Current Issue
    Methylation-sensitive Amplified Polymorphism as a Tool to Analyze Wild Potato Hybrids
    Authors:  Nicolás Cara, Carlos F. Marfil, María V. Bertoldi and Ricardo W. Masuelli, date: 07/05/2020, view: 481, Q&A: 0
    [Abstract] Methylation-Sensitive Amplification Polymorphism (MSAP) is a versatile marker for analyzing DNA methylation patterns in non-model species. The implementation of this technique does not require a reference genome and makes it possible to determine the methylation status of hundreds of anonymous loci distributed throughout the genome. In addition, ...
    Analysis of Replicative Intermediates of Adeno-associated Virus through Hirt Extraction and Southern Blotting
    [Abstract] Adeno-associated virus (AAV) is a small single-stranded DNA virus that requires the presence of a helper virus, such as adenovirus or herpes virus, to efficiently replicate its genome. AAV DNA is replicated by a rolling-hairpin mechanism (Ward, 2006), and during replication several DNA intermediates can be detected. This detailed protocol ...
    Capillary Electrophoresis in Hydroxyethylcellulose Solutions for the Analysis of dsDNA, dsRNA, and siRNA
    Authors:  Chenchen Liu, Yoshinori Yamaguchi and Xiaoming Dou, date: 08/05/2016, view: 6081, Q&A: 0
    [Abstract] Capillary electrophoresis (CE) is identified as a promising technology for the study of nucleic acid molecules because of its high efficiency, high throughput with automation and integration. Compared to the traditional method of slab gel electrophoresis (SGE), the advantages of CE cannot be emphasized more. Most of CE process, including sample ...
    DNA Fragmentation Analysis
    Authors:  Kei-ichiro Mishiba, Yukihiro Nagashima, Noriko Hayashi and Nozomu Koizumi, date: 08/05/2014, view: 13270, Q&A: 0
    [Abstract] DNA fragmentation with length corresponding to multiple integer of approximately 180 base pairs is a distinct feature of apoptosis in animals and programmed cell death in plants. This feature can simply be detected by DNA gel electrophoresis followed by ethidium bromide staining, although in some cases it is difficult to distinguish the DNA ...
    A Quick, No Frills Approach to Mouse Genotyping
    Author:  Manuel E. Lopez, date: 08/05/2012, view: 22097, Q&A: 1
    [Abstract] Mice are extremely powerful mammalian genetic model organisms for basic and medical research, but managing a colony of transgenic mice is time consuming and expensive, many times requiring the help of dedicated technicians. Slow and laborious genotyping procedures add to the hassle. Outsourcing is costly and may not be as fast as desired, ...
    Yeast DNA Replication 2D Gel Protocol
    Author:  Yanfei Zou, date: 07/05/2012, view: 12238, Q&A: 0
    [Abstract] Two-dimensional agarose gel electrophoresis (2D gel) analysis is used extensively as a method to detect origins of replication. Here, I present a simplified method for the isolation of yeast genomic DNA for 2D gel analysis from a small number of yeast cells. This DNA isolation method is simpler and less time consuming than the traditional method ...



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