Biochemistry

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    Protocols in Current Issue
    FACS-based Glucose Uptake Assay of Mouse Embryonic Fibroblasts and Breast Cancer Cells Using 2-NBDG Probe
    Authors:  Shengli Dong and Suresh K Alahari, date: 04/20/2018, view: 4207, Q&A: 0
    [Abstract] This is a flow cytometry-based protocol to measure glucose uptake of mouse embryonic fibroblasts (MEFs) and breast cancer cells in vitro. The method is a slightly modified and updated version as previously described (Dong et al., 2017). Briefly, the target cells are incubated with the fluorescently tagged ...
    Determination of Intra- and Extracellular Glucose in Mycelium of Fusarium oxysporum
    Authors:  Carmen Ruiz-Roldan and M. Isabel G. Roncero, date: 07/20/2016, view: 6294, Q&A: 0
    [Abstract] To study alterations in the metabolism and/or in the transport of glucose during Fusarium oxysporum vegetative growth, we determined intracellular glucose levels in different fungal strains, as well as the amount of glucose remaining in the supernatants after growth in synthetic medium (SM) supplemented with either 0.05 or 2.5% glucose. ...
    13C Tracer Studies of Metabolism in Mouse Tumor Xenografts
    Authors:  Andrew N. Lane, Jun Yan and Teresa W-M. Fan, date: 11/20/2015, view: 7343, Q&A: 0
    [Abstract] Mice are widely used for human tumor xenograft studies of cancer development and drug efficacy and toxicity. Stable isotope tracing coupled with metabolomic analysis is an emerging approach for assaying metabolic network activity. In mouse models there are several routes of tracer introduction, which have particular advantages and disadvantages ...
    Analysis of Malondialdehyde, Chlorophyll Proline, Soluble Sugar, and Glutathione Content in Arabidopsis seedling
    Authors:  Zhijin Zhang and Rongfeng Huang, date: 07/20/2013, view: 22448, Q&A: 2
    [Abstract] The protocol has four sub-protocols, which are about the measurement of malondialdehyde, chlorophyll proline, soluble sugar, and glutathione content, respectively, in Arabidopsis seedling by using spectrophotometer. These methods are simple, effective and reproducible, which will help the researchers who are not familiar with these ...
    Glucose Production Assay in Primary Mouse Hepatocytes
    Authors:  Michihiro Matsumoto and Mashito Sakai, date: 11/05/2012, view: 19263, Q&A: 3
    [Abstract] Hepatic glucose production is a primary determinant of fasting hyperglycemia in type 2 diabetic patients. Glucagon-cAMP-PKA pathway increases, but insulin-PI3 kinase-Akt pathway suppresses glucose production. This assay aims to evaluate the ability of isolated mouse hepatocytes to release newly synthesized glucose mainly from lactate and pyruvate ...
    5-Hydroxymethylcytosine (5-hmC) Specific Enrichment
    Authors:  Keith E Szulwach, Chun-Xiao Song, Chuan He and Peng Jin, date: 08/05/2012, view: 7549, Q&A: 0
    [Abstract] 5-Hydroxymethylcytosine (5-hmC) is a newly discovered DNA modification in mammalian genomes. This protocol is to be a highly efficient and selective chemical approach to label and capture 5-hmC, taking advantage of a bacteriophage enzyme that adds a glucose moiety to 5-hmC specifically, which could in turn be used for high-throughput mapping via ...



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