Molecular Biology

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    Protocols in Current Issue
    In vitro Enzymatic Assays of Histone Decrotonylation on Recombinant Histones
    Authors:  Rachel Fellows and Patrick Varga-Weisz, date: 07/20/2018, view: 2279, Q&A: 0
    [Abstract] Class I histone deacetylases (HDACs) are efficient histone decrotonylases, broadening the enzymatic spectrum of these important (epi-)genome regulators and drug targets. Here, we describe an in vitro approach to assaying class I HDACs with different acyl-histone substrates, including crotonylated histones and expand this to examine the ...



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