Biochemistry

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    Protocols in Current Issue
    Determination of Polyhydroxybutyrate (PHB) Content in Ralstonia eutropha Using Gas Chromatography and Nile Red Staining
    Authors:  Janina R. Juengert, Stephanie Bresan and Dieter Jendrossek, date: 03/05/2018, view: 12024, Q&A: 0
    [Abstract] Ralstonia eutropha H16 produces and mobilizes (re-utilizes) intracellular polyhydroxybutyrate (PHB) granules during growth. This protocol describes the visualization of intracellular Nile red stained PHB granules and the quantification of PHB by gas chromatography. Our first method describes how to analyze PHB granules by fluorescence ...
    Quantitative Determination of Poly-β-hydroxybutyrate in Synechocystis sp. PCC 6803
    Authors:  Yvonne Zilliges and Ramon Damrow, date: 07/20/2017, view: 6825, Q&A: 0
    [Abstract] Cyanobacteria synthesize a variety of chemically-different, high-value biopolymers such as glycogen (polyglucose), poly-β-hydroxybutyrate (PHB), cyanophycin (polyamide of arginine and aspartic acid) and volutin (polyphosphate) under excess conditions. Especially under unbalanced C to N ratios, glycogen and in some cyanobacterial genera ...



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