Cell Biology


    Protocols in Current Issue
    Method for Measuring Mucociliary Clearance and Cilia-generated Flow in Mice by ex vivo Imaging
    Authors:  Eric Song and Akiko Iwasaki, date: 03/20/2020, view: 682, Q&A: 0
    [Abstract] Ex vivo biophysical measurements provide valuable insights into understanding both physiological and pathogenic processes. One critical physiological mechanism that is regulated by these biophysical properties is cilia-generated flow that mediates mucociliary clearance, which is known to provide protection against foreign particles and ...
    Characterization of Biological Motion Using Motion Sensing Superpixels
    Authors:  Felix Y. Zhou, Carlos Ruiz-Puig, Richard P. Owen, Michael J. White, Jens Rittscher and Xin Lu, date: 09/20/2019, view: 1541, Q&A: 0
    [Abstract] Precise spatiotemporal regulation is the foundation for the healthy development and maintenance of living organisms. All cells must correctly execute their function in the right place at the right time. Cellular motion is thus an important dynamic readout of signaling in key disease-relevant molecular pathways. However despite the rapid ...
    A Novel Technique for Imaging and Analysis of Hair Cells in the Organ of Corti Using Modified Sca/eS and Machine Learning
    [Abstract] Here, we describe a sorbitol-based optical clearing method, called modified Sca/eS that can be used to image all hair cells (HCs) in the mouse cochlea. This modification of Sca/eS is defined by three steps: decalcification, de-lipidation, and refractive index matching, which can all be completed within 72 h. Furthermore, we established automated ...
    Looking through Brains with Fast Passive CLARITY: Zebrafish, Rodents, Non-human Primates and Humans
    Authors:  Farzad Mortazavi, Alexander J. Stankiewicz and Irina V. Zhdanova, date: 08/05/2019, view: 2927, Q&A: 0
    [Abstract] Recently developed CLARITY (Clear Lipid-exchanged Acrylamide-hybridized Rigid Imaging/Immunostaining/In situ-hybridization-compatible Tis-sue-hYdrogel) technique renders the tissue transparent by removing lipids in the tissue, while preserving and stabilizing the cellular and subcellular structures. This provides effective penetration of diverse ...
    Immunofluorescence Staining of WT-1/Podocalyxin on Mouse Kidney Sections
    Authors:  Carole Henique, Olivia Lenoir and Pierre-Louis Tharaux, date: 04/20/2019, view: 2212, Q&A: 0
    [Abstract] In glomerular diseases, podocytes are one type of cells involved in dysfunction of glomerular filtration. In these conditions, podocyte proteins expression change. Therefore, immunofluorescent staining of podocytes can be performed to visualize podocyte localization of proteins of interest. In this protocol, we detail a method to stain podocytes ...
    A Method for Culturing Mouse Whisker Follicles to Study Circadian Rhythms ex vivo
    Authors:  Atsuhiro Nishida, Yoshiki Miyawaki, Koichi Node and Makoto Akashi, date: 01/20/2019, view: 2136, Q&A: 0
    [Abstract] Ex vivo tissue-culture experiments are often performed in the field of circadian biology. The major aim of these experiments is to evaluate circadian characteristics such as period length at the tissue-autonomous level by monitoring clock gene expression in real time. This culture method is also used to examine the tissue specificity of ...
    An Image Analysis Pipeline to Quantify Emerging Cracks in Materials or Adhesion Defects in Living Tissues
    Authors:  Stéphane Verger, Guillaume Cerutti and Olivier Hamant, date: 10/05/2018, view: 2825, Q&A: 0
    [Abstract] Microcracks in materials reflect their mechanical properties. The quantification of the number or orientation of such cracks is thus essential in many fields, including engineering and geology. In biology, cracks in soft tissues can reflect adhesion defects, and the analysis of their pattern can help to deduce the magnitude and orientation of ...
    A Method to Injure, Dissect and Image Indirect Flight Muscle of Drosophila
    Authors:  Kunal Chakraborty, K. VijayRaghavan and Rajesh Gunage, date: 05/20/2018, view: 4650, Q&A: 0
    [Abstract] Inducing an injury specifically to Drosophila flight muscles is a difficult task, owing to the small size of the muscles and the presence of the cuticle. The protocol described below provides an easy and reproducible method to induce injury in the Drosophila flight muscles.
    Quantification of Bacterial Attachment to Tissue Sections
    Authors:  Batya Isaacson, Tehila Hadad, Gilad Bachrach and Ofer Mandelboim, date: 03/05/2018, view: 4410, Q&A: 0
    [Abstract] Here we describe a method to test bacterial adhesion to paraffin embedded tissue sections. This method allows examining binding of different bacterial strains, transfected with a fluorescent protein reporter plasmid to various tissues, to better understand different mechanisms such as colonization. This assay provides a more physiological context ...
    Obtaining Acute Brain Slices
    Authors:  Thomas Papouin and Philip G. Haydon, date: 01/20/2018, view: 9882, Q&A: 1
    [Abstract] Obtaining acute brain slices for electrophysiology or amperometric recordings has become a routine procedure in most labs in the field of neuroscience. Yet, protocols describing the step by step process are scarce, in particular for routine acute preparations such as from the mouse hippocampus. Here we provide a detailed protocol for the ...

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