Protocols in Current Issue
    Identification of Socially-activated Neurons
    Authors:  Mary L. Phillips and Lucas Pozzo-Miller, date: 09/05/2020, view: 2566, Q&A: 0
    [Abstract] Determining the neuronal circuitry responsible for specific behaviors is a major focus in the field of neurobiology. Activity-dependent immediate early genes (IEGs), transcribed and translated shortly after neurons discharge action potentials, have been used extensively to either identify or gain genetic access to neurons and brain regions ...
    High-resolution Immunoelectron Microscopy Techniques for Revealing Distinct Subcellular Type 1 Cannabinoid Receptor Domains in Brain
    [Abstract] Activation of type 1 cannabinoid (CB1) receptors by endogenous, exogenous (cannabis derivatives) or synthetic cannabinoids (i.e., CP 55.940, Win-2) has a wide variety of behavioral effects due to the presence of CB1 receptors in the brain. In situ hybridization and immunohistochemical techniques have been ...
    An ex vivo Perifusion Method for Quantitative Determination of Neuropeptide Release from Mouse Hypothalamic Explants
    Authors:  Ophélia Le Thuc, Jacques Noël and Carole Rovère, date: 08/20/2017, view: 5741, Q&A: 0
    [Abstract] The hypothalamus is a primary brain area which, in mammals, regulates several physiological functions that are all related to maintaining general homeostasis, by linking the central nervous system (CNS) and the periphery. The hypothalamus itself can be considered an endocrine brain region of some sort as it hosts in its different nuclei several ...
    Axonal Conduction Velocity Measurement
    Authors:  Margaret Louise DeMaegd, Carola Städele and Wolfgang Stein, date: 03/05/2017, view: 8973, Q&A: 0
    [Abstract] Action potential conduction velocity is the speed at which an action potential (AP) propagates along an axon. Measuring AP conduction velocity is instrumental in determining neuron health, function, and computational capability, as well as in determining short-term dynamics of neuronal communication and AP initiation (Ballo and Bucher, 2009; ...

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