Cancer Biology


    Protocols in Current Issue
    Cell Synchronization by Double Thymidine Block
    Authors:  Guo Chen and Xingming Deng, date: 09/05/2018, view: 4945, Q&A: 7
    [Abstract] Cell synchronization is widely used in studying mechanisms involves in regulation of cell cycle progression. Through synchronization, cells at distinct cell cycle stage could be obtained. Thymidine is a DNA synthesis inhibitor that can arrest cell at G1/S boundary, prior to DNA replication. Here, we present the protocol to synchronize cells at ...
    Quantitative Live-cell Reporter Assay for Noncanonical Wnt Activity
    Authors:  Edith P. Karuna, Michael W. Susman and Hsin-Yi Henry Ho, date: 03/20/2018, view: 2380, Q&A: 0
    [Abstract] Noncanonical Wnt signaling functions independently of the β-catenin pathway to control diverse developmental processes, and dysfunction of the pathway contributes to a number of human pathological conditions, including birth defects and metastatic cancer. Progress in the field, however, has been hampered by the scarcity of functional assays for ...
    Rapid Profiling Cell Cycle by Flow Cytometry Using Concurrent Staining of DNA and Mitotic Markers
    Authors:  Yuqing Shen, Paolo Vignali and Ruoning Wang, date: 08/20/2017, view: 3814, Q&A: 0
    [Abstract] The flow cytometric quantitation of DNA content by DNA-binding fluorochrome, propidium iodide (PI) is the most widely used method for cell cycle analysis. However, the commonly used methods are time-consuming and labor-intensive and are incompatible with staining of mitotic markers by fluorescent-labeled antibodies. Here, we report an optimized ...

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