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Real-time Three-dimensional Tracking of Endocytic Vesicles

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A Standard Operative Procedure for Safe-handling of Remains and Wastes of COVID-19 Patients

Authors: Ravi Kant Narayan, Adil Asghar, Rakesh Parashar, Chiman Kumari, Kamla Kant, Ashok Rastogi and Ashutosh Kumar, date: 10/20/2020, view: 153, Q&A: 0

The ongoing coronavirus disease-2019 (COVID-19) pandemic has raised significant public health issues which need to be attended. To enable efficient handling of the situation and prevent the spread of the epidemic, healthcare professionals require ...

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Site-specific Labeling of B Cell Receptor and Soluble Immunoglobulin

Authors: Yu Wang, Zhixun Shen, Zhengpeng Wan and Wanli Liu, date: 09/20/2020, view: 445, Q&A: 0

[Abstract] B lymphocyte activation is regulated by its membrane-bound B cell receptors (BCRs) upon recognizing diverse antigens. It is hypothesized that antigen binding would trigger conformational changes within BCRs, followed by a series of downstream signaling activation. To measure the BCR conformational changes in live cells, a fluorescent site-specific ...

Surgical Induction of Endometriosis in Female Mice

Authors: Alejandra Escudero-Lara, David Cabañero and Rafael Maldonado, date: 09/20/2020, view: 356, Q&A: 0

[Abstract] Endometriosis is a common gynecological disease characterized by the presence of endometrial tissue outside the uterine cavity. It is frequently associated with pain, infertility and a reduced quality of life, and it lacks adequate treatment. Several rodent models of endometriosis have been developed through heterologous and homologous ...

Immunohistochemistry of Kidney a-SMA, Collagen 1, and Collagen 3, in A Novel Mouse Model of Reno-cardiac Syndrome

Authors: Julius E. Kieswich, Jianmin Chen, Samira Alliouachene, Paul W. Caton, Kieran McCafferty, Christoph Thiemermann and Muhammad M. Yaqoob, date: 09/20/2020, view: 329, Q&A: 0

[Abstract] Cardiorenal syndrome defines a synergistic pathology of the heart and kidneys where failure of one organ causes failure in the other. The incidence of cardiovascular mortality caused by this syndrome, is 20 fold higher in the end stage renal disease (ESRD) population compared to the population as a whole thus necessitating the need for improved ...

Flow-cytometric Detection of Low-level Reactive Oxygen Species in Cell Lines and Primary Immune Cells

Authors: Kevin Bode, Corinna Link, Peter H. Krammer and Heiko Weyd, date: 09/05/2020, view: 1084, Q&A: 0

[Abstract] Depending on its concentration and cellular origin the production of reactive oxygen species (ROS) in the organism serves a variety of functions. While high concentrations during an oxidative burst are used to fight pathogens, low to moderate amounts of ROS act as signaling molecules important for several physiological processes such as regulation ...

Optogenetic Tuning of Protein-protein Binding in Bilayers Using LOVTRAP

Authors: Doug Tischer and Orion D. Weiner, date: 09/05/2020, view: 851, Q&A: 0

[Abstract] Modern microscopy methods are powerful tools for studying live cell signaling and biochemical reactions, enabling us to observe when and where these reactions take place from the level of a cell down to single molecules. With microscopy, each cell or molecule can be observed both before and after a given perturbation, facilitating better inference ...

Multiplication and Growth Inhibition Activity Assays for the Zoonotic Malaria Parasite, Plasmodium knowlesi

Authors: Franziska Mohring, Thomas A. Rawlinson, Simon J. Draper and Robert W. Moon, date: 09/05/2020, view: 621, Q&A: 0

[Abstract] Malaria remains a major cause of morbidity and mortality globally. Clinical symptoms of the disease arise from the growth and multiplication of Plasmodium parasites within the blood of the host. Thus in vitro assays to determine how drug, antibody and genetic perturbations affect the growth rate of Plasmodium parasites ...

Real-time in vivo Imaging of LPS-induced Local Inflammation and Drug Deposition in NF-κB Reporter Mice

Authors: Artur Schmidtchen and Manoj Puthia, date: 08/20/2020, view: 966, Q&A: 0

[Abstract] Wound, biomaterial, and surgical infections are all characterized by a localized and excessive inflammation, motivating the development of in vivo methods focused on the analysis of local immune events. However, current inflammation models, such as the commonly used in vivo models of endotoxin-induced inflammation are based on ...

Neutrophil Extracellular Trap Killing Assay of Candida albicans

Authors: Sheng-Yang Wu and Betty A. Wu-Hsieh, date: 08/20/2020, view: 857, Q&A: 0

[Abstract] Fungal pathogen Candida albicans is one of the top leading causes of overall healthcare-associated bloodstream infections worldwide. Neutrophil is the major effector cell to clear C. albicans infection. Our study showed that mouse neutrophils utilize two independent mechanisms to kill C. albicans: one is CR3 downstream ...

Isolation and Quantification of Extracellular DNA from Biofluids

Authors: Ľubica Janovičová, Barbora Konečná, Barbora Vlková and Peter Celec, date: 08/20/2020, view: 763, Q&A: 0

[Abstract] Extracellular DNA is studied as a diagnostic biomarker, but also as a factor involved in the pathophysiology of several diseases due to its pro-inflammatory properties. Extracellular DNA can be extracted from plasma, urine, saliva or other biofluids using standard DNA isolation procedures and specialized commercial kits. Sample preparation for ...

Flow Cytometry of CD14, VDR, Cyp27 and Cyp24 and TLR4 in U937 Cells

Authors: Jacqueline Ferritto Rebello, Rodrigo Barbosa de Oliveira Brito, Caren Cristina Grabulosa, Rosa Maria Affonso Moyses, Rosilene Motta Elias and Maria Aparecida Dalboni, date: 08/05/2020, view: 766, Q&A: 0

[Abstract] Chronic Kidney Disease (CKD) patients present a micro inflammation state due to failure renal function. The calcitriol has been described as an anti-inflammatory factor that might modulates the inflammatory response in CKD patients. However, these patients have deficiency of Calcitriol due to failure renal function. But, synthesis of this vitamin ...

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