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Principal Investigator Research interests
Cancer, immunotherapy, immune monitoring, T cell, CMV, CyTOF, flow cytometry
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Lab Introduction Holden Maecker is an Associate Professor of Microbiology and Immunology at Stanford University. His research group is focused on cellular immune responses to chronic pathogens and cancer, and the correlation of those responses with immune protection.

The group has published a number of papers on this topic, both independently and in collaboration with other groups, in systems such as CMV, HIV, tuberculosis, and cancer.

Dr. Maecker also directs the Human Immune Monitoring Center (HIMC), a Stanford core service laboratory, which performs blood processing and standardized assays using flow cytometry, genomics, and immunoassay platforms.

Since 2010, his lab has gained extensive experience with CyTOF mass cytometry.
Selected peer-reviewed publications
  1. Shen-Orr, S. S., Furman, D., Kidd, B. A., Hadad, F., Lovelace, P., Huang, Y. W., Rosenberg-Hasson, Y., Mackey, S., Grisar, F. A., Pickman, Y., Maecker, H. T., Chien, Y. H., Dekker, C. L., Wu, J. C., Butte, A. J. and Davis, M. M. (2016). Defective Signaling in the JAK-STAT Pathway Tracks with Chronic Inflammation and Cardiovascular Risk in Aging Humans. Cell Syst 3(4): 374-384 e374.
  2. Zaunders, J., Jing, J., Leipold, M., Maecker, H., Kelleher, A. D. and Koch, I. (2016). Computationally efficient multidimensional analysis of complex flow cytometry data using second order polynomial histograms. Cytometry A 89(1): 44-58.
  3. Mukai, K., Gaudenzio, N., Gupta, S., Vivanco, N., Bendall, S. C., Maecker, H. T., Chinthrajah, R. S., Tsai, M., Nadeau, K. C. and Galli, S. J. (2016). Assessing basophil activation by using flow cytometry and mass cytometry in blood stored 24 hours before analysis. J Allergy Clin Immunol. [Epub ahead of print]
  4. Japp, A. S., Hoffmann, K., Schlickeiser, S., Glauben, R., Nikolaou, C., Maecker, H. T., Braun, J., Matzmohr, N., Sawitzki, B., Siegmund, B., Radbruch, A., Volk, H. D., Frentsch, M., Kunkel, D. and Thiel, A. (2016). Wild immunology assessed by multidimensional mass cytometry. Cytometry A. [Epub ahead of print]
  5. Yabu, J. M., Siebert, J. C. and Maecker, H. T. (2016). Immune Profiles to Predict Response to Desensitization Therapy in Highly HLA-Sensitized Kidney Transplant Candidates. PLoS One 11(4): e0153355.
  6. Ryan, J. F., Hovde, R., Glanville, J., Lyu, S. C., Ji, X., Gupta, S., Tibshirani, R. J., Jay, D. C., Boyd, S. D., Chinthrajah, R. S., Davis, M. M., Galli, S. J., Maecker, H. T. and Nadeau, K. C. (2016). Successful immunotherapy induces previously unidentified allergen-specific CD4+ T-cell subsets. Proc Natl Acad Sci U S A 113(9): E1286-1295.
  7. Finak, G., Langweiler, M., Jaimes, M., Malek, M., Taghiyar, J., Korin, Y., Raddassi, K., Devine, L., Obermoser, G., Pekalski, M. L., Pontikos, N., Diaz, A., Heck, S., Villanova, F., Terrazzini, N., Kern, F., Qian, Y., Stanton, R., Wang, K., Brandes, A., Ramey, J., Aghaeepour, N., Mosmann, T., Scheuermann, R. H., Reed, E., Palucka, K., Pascual, V., Blomberg, B. B., Nestle, F., Nussenblatt, R. B., Brinkman, R. R., Gottardo, R., Maecker, H. and McCoy, J. P. (2016). Standardizing Flow Cytometry Immunophenotyping Analysis from the Human ImmunoPhenotyping Consortium. Sci Rep 6: 20686.
  8. Hiniker, S. M., Reddy, S. A., Maecker, H. T., Subrahmanyam, P. B., Rosenberg-Hasson, Y., Swetter, S. M., Saha, S., Shura, L. and Knox, S. J. (2016). A Prospective Clinical Trial Combining Radiation Therapy With Systemic Immunotherapy in Metastatic Melanoma. Int J Radiat Oncol Biol Phys 96(3): 578-588.
  9. Kojima, N., Bristow, C. C., Maecker, H., Rosenberg-Hasson, Y., Leon, S. R., Vargas, S. K., Konda, K. A., Caceres, C. F. and Klausner, J. D. (2016). Similarities in the Markers of Inflammation Between Men With Syphilis and Women With Increased Risk of HIV Acquisition. Clin Infect Dis 62(2): 265-266.
  10. Kuznetsova, T., Haddad, F., Knez, J., Rosenberg-Hasson, Y., Sung, J., Cauwenberghs, N., Thijs, L., Karakikes, I., Maecker, H., Mahaffey, K. W., Wu, J. C. and Staessen, J. A. (2015). Cytokines profile in hypertensive patients with left ventricular remodeling and dysfunction. J Am Soc Hypertens 9(12): 975-984 e973.
  11. Mei, H. E., Leipold, M. D. and Maecker, H. T. (2016). Platinum-conjugated antibodies for application in mass cytometry. Cytometry A 89(3): 292-300.
  12. Yuan, J., Hegde, P. S., Clynes, R., Foukas, P. G., Harari, A., Kleen, T. O., Kvistborg, P., Maccalli, C., Maecker, H. T., Page, D. B., Robins, H., Song, W., Stack, E. C., Wang, E., Whiteside, T. L., Zhao, Y., Zwierzina, H., Butterfield, L. H. and Fox, B. A. (2016). Novel technologies and emerging biomarkers for personalized cancer immunotherapy. J Immunother Cancer 4: 3.
  13. Maecker, H. T. (2016). New Cellular Assays.  In: Nathanson, N. (Ed.). Viral Pathogenesis, Elsevier Ltd, pp. 315-317.
  14. Whiting, C. C., Siebert, J., Newman, A. M., Du, H. W., Alizadeh, A. A., Goronzy, J., Weyand, C. M., Krishnan, E., Fathman, C. G. and Maecker, H. T. (2015). Large-Scale and Comprehensive Immune Profiling and Functional Analysis of Normal Human Aging. PLoS One 10(7): e0133627.
  15. Haddad, F., Sever, M., Poglajen, G., Lezaic, L., Yang, P., Maecker, H., Davis, M., Kuznetsova, T., Wu, J. C. and Vrtovec, B. (2015). Immunologic Network and Response to Intramyocardial CD34+ Stem Cell Therapy in Patients With Dilated Cardiomyopathy. J Card Fail 21(7): 572-582.
  16. Furman, D., Jojic, V., Sharma, S., Shen-Orr, S. S., Angel, C. J., Onengut-Gumuscu, S., Kidd, B. A., Maecker, H. T., Concannon, P., Dekker, C. L., Thomas, P. G. and Davis, M. M. (2015). Cytomegalovirus infection enhances the immune response to influenza. Sci Transl Med 7(281): 281ra243.
  17. Mei, H. E., Leipold, M. D., Schulz, A. R., Chester, C. and Maecker, H. T. (2015). Barcoding of live human peripheral blood mononuclear cells for multiplexed mass cytometry. J Immunol 194(4): 2022-2031.
  18. Brodin, P., Jojic, V., Gao, T., Bhattacharya, S., Angel, C. J., Furman, D., Shen-Orr, S., Dekker, C. L., Swan, G. E., Butte, A. J., Maecker, H. T. and Davis, M. M. (2015). Variation in the human immune system is largely driven by non-heritable influences. Cell 160(1-2): 37-47.
  19. Courtot, M., Meskas, J., Diehl, A. D., Droumeva, R., Gottardo, R., Jalali, A., Taghiyar, M. J., Maecker, H. T., McCoy, J. P., Ruttenberg, A., Scheuermann, R. H. and Brinkman, R. R. (2015). flowCL: ontology-based cell population labelling in flow cytometry. Bioinformatics 31(8): 1337-1339.
  20. Kvistborg, P., Gouttefangeas, C., Aghaeepour, N., Cazaly, A., Chattopadhyay, P. K., Chan, C., Eckl, J., Finak, G., Hadrup, S. R., Maecker, H. T., Maurer, D., Mosmann, T., Qiu, P., Scheuermann, R. H., Welters, M. J., Ferrari, G., Brinkman, R. R. and Britten, C. M. (2015). Thinking outside the gate: single-cell assessments in multiple dimensions. Immunity 42(4): 591-592.
  21. Hiniker, S. M., Maecker, H. T. and Knox, S. J. (2015). Predictors of clinical response to immunotherapy with or without radiotherapy. J Radiat Oncol 4: 339-345.
  22. Maecker, H. T. and Harari, A. (2015). Immune monitoring technology primer: flow and mass cytometry. J Immunother Cancer 3: 44.
  23. Chester, C. and Maecker, H. T. (2015). Algorithmic Tools for Mining High-Dimensional Cytometry Data. J Immunol 195(3): 773-779.
  24. Leipold, M. D., Newell, E. W. and Maecker, H. T. (2015). Multiparameter Phenotyping of Human PBMCs Using Mass Cytometry. Methods Mol Biol 1343: 81-95.
  25. Nair, N., Mei, H. E., Chen, S. Y., Hale, M., Nolan, G. P., Maecker, H. T., Genovese, M., Fathman, C. G. and Whiting, C. C. (2015). Mass cytometry as a platform for the discovery of cellular biomarkers to guide effective rheumatic disease therapy. Arthritis Res Ther 17: 127.
  26. Mannick, J. B., Del Giudice, G., Lattanzi, M., Valiante, N. M., Praestgaard, J., Huang, B., Lonetto, M. A., Maecker, H. T., Kovarik, J., Carson, S., Glass, D. J. and Klickstein, L. B. (2014). mTOR inhibition improves immune function in the elderly. Sci Transl Med 6(268): 268ra179.
  27. Nadeau, K. C., Li, Z., Farzan, S., Koestler, D., Robbins, D., Fei, D. L., Malipatlolla, M., Maecker, H., Enelow, R., Korrick, S. and Karagas, M. R. (2014). In utero arsenic exposure and fetal immune repertoire in a US pregnancy cohort. Clin Immunol 155(2): 188-197.
  28. DiCarlo, J., Agarwal-Hashmi, R., Shah, A., Kim, P., Craveiro, L., Killen, R., Rosenberg-Hasson, Y. and Maecker, H. (2014). Cytokine and chemokine patterns across 100 days after hematopoietic stem cell transplantation in children. Biol Blood Marrow Transplant 20(3): 361-369.
  29. Rosenberg-Hasson, Y., Hansmann, L., Liedtke, M., Herschmann, I. and Maecker, H. T. (2014). Effects of serum and plasma matrices on multiplex immunoassays. Immunol Res 58(2-3): 224-233.
  30. Chang, S., Kohrt, H. and Maecker, H. T. (2014). Monitoring the immune competence of cancer patients to predict outcome. Cancer Immunol Immunother 63(7): 713-719.
  31. Leipold, M. and Maecker, H. T. (2013). Multiparameter phenotyping of human PBMCs using mass cytometry. In: Shaw, A. (ed). Immunosenescence.
  32. Furman, D., Jojic, V., Kidd, B., Shen-Orr, S., Price, J., Jarrell, J., Tse, T., Huang, H., Lund, P., Maecker, H. T., Utz, P. J., Dekker, C. L., Koller, D. and Davis, M. M. (2013). Apoptosis and other immune biomarkers predict influenza vaccine responsiveness. Mol Syst Biol 9: 659.
  33. Horowitz, A., Strauss-Albee, D. M., Leipold, M., Kubo, J., Nemat-Gorgani, N., Dogan, O. C., Dekker, C. L., Mackey, S., Maecker, H., Swan, G. E., Davis, M. M., Norman, P. J., Guethlein, L. A., Desai, M., Parham, P. and Blish, C. A. (2013). Genetic and environmental determinants of human NK cell diversity revealed by mass cytometry. Sci Transl Med 5(208): 208ra145.
  34. Britten, C. M., Janetzki, S., Butterfield, L. H., Ferrari, G., Gouttefangeas, C., Huber, C., Kalos, M., Levitsky, H. I., Maecker, H. T., Melief, C. J., O'Donnell-Tormey, J., Odunsi, K., Old, L. J., Ottenhoff, T. H., Ottensmeier, C., Pawelec, G., Roederer, M., Roep, B. O., Romero, P., van der Burg, S. H., Walter, S., Hoos, A. and Davis, M. M. (2012). T cell assays and MIATA: the essential minimum for maximum impact. Immunity 37(1): 1-2.
  35. Leipold, M. D. and Maecker, H. T. (2012). Mass cytometry: protocol for daily tuning and running cell samples on a CyTOF mass cytometer. J Vis Exp(69): e4398.
  36. Maecker, H. T., McCoy, J. P. and Nussenblatt, R. (2012). Standardizing immunophenotyping for the Human Immunology Project. Nat Rev Immunol 12(3): 191-200.
  37. Siebert, J. C., Munsil, W., Rosenberg-Hasson, Y., Davis, M. M. and Maecker, H. T. (2012). The Stanford Data Miner: a novel approach for integrating and exploring heterogeneous immunological data. J Transl Med 10: 62.
  38. Streitz, M., Fuhrmann, S., Thomas, D., Cheek, E., Nomura, L., Maecker, H., Martus, P., Aghaeepour, N., Brinkman, R. R., Volk, H. D. and Kern, F. (2012). The phenotypic distribution and functional profile of tuberculin-specific CD4 T-cells characterizes different stages of TB infection. Cytometry B Clin Cytom 82(6): 360-368.
  39. Streitz, M., Fuhrmann, S., Powell, F., Quassem, A., Nomura, L., Maecker, H., Martus, P., Volk, H. D. and Kern, F. (2011). Tuberculin-specific T cells are reduced in active pulmonary tuberculosis compared to LTBI or status post BCG vaccination. J Infect Dis 203(3): 378-382.
  40. Jaimes, M. C., Maecker, H. T., Yan, M., Maino, V. C., Hanley, M. B., Greer, A., Darden, J. M. and D'Souza, M. P. (2011). Quality assurance of intracellular cytokine staining assays: analysis of multiple rounds of proficiency testing. J Immunol Methods 363(2): 143-157.
  41. Maecker, H. T., McCoy, J. P., Jr., Consortium, F. H. I., Amos, M., Elliott, J., Gaigalas, A., Wang, L., Aranda, R., Banchereau, J., Boshoff, C., Braun, J., Korin, Y., Reed, E., Cho, J., Hafler, D., Davis, M., Fathman, C. G., Robinson, W., Denny, T., Weinhold, K., Desai, B., Diamond, B., Gregersen, P., Di Meglio, P., Nestle, F. O., Peakman, M., Villanova, F., Ferbas, J., Field, E., Kantor, A., Kawabata, T., Komocsar, W., Lotze, M., Nepom, J., Ochs, H., O'Lone, R., Phippard, D., Plevy, S., Rich, S., Roederer, M., Rotrosen, D. and Yeh, J. H. (2010). A model for harmonizing flow cytometry in clinical trials. Nat Immunol 11(11): 975-978.
  42. Janetzki, S., Britten, C. M., Kalos, M., Levitsky, H. I., Maecker, H. T., Melief, C. J., Old, L. J., Romero, P., Hoos, A. and Davis, M. M. (2009). "MIATA"-minimal information about T cell assays. Immunity 31(4): 527-528.