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    Assessment of Aversion of Acute Pain Stimulus through Conditioned Place Aversion
    Pain is a complex experience. The aversive component of pain has been assessed through conditioned place aversion in rodents. However, this behavioral test does not allow the evaluation of the aversion of an acute pain stimulus. In Zhang et al. ...
    Isolation, Culture and Differentiation of Adult Hippocampal Precursor Cells
    Authors:  Stefanie N. Bernas, Odette Leiter, Tara L. Walker and Gerd Kempermann, date: 11/05/2017, view: 370, Q&A: 0
    There are two neurogenic niches in the adult mammalian brain: the subventricular zone of the lateral ventricle and the subgranular zone of the hippocampal dentate gyrus. Cells from these areas can be isolated and maintained in vitro, using ...
    Sensitive Estimation of Flavor Preferences in STFP Using Cumulative Time Profiles
    Authors:  Aditya Singh and J. Balaji, date: 11/05/2017, view: 215, Q&A: 0
    Social transmission of food preference (STFP) is observed among rodents between a demonstrator and a naïve hungry observer. During social interaction, hungry observer receives information about safety of the food consumed by the demonstrator. This ...
    Assessment of Aversion of Acute Pain Stimulus through Conditioned Place Aversion
    [Abstract] Pain is a complex experience. The aversive component of pain has been assessed through conditioned place aversion in rodents. However, this behavioral test does not allow the evaluation of the aversion of an acute pain stimulus. In Zhang et al. (2017), we provide an updated version of a Conditioned Place Aversion paradigm to address this ...
    Isolation, Culture and Differentiation of Adult Hippocampal Precursor Cells
    Authors:  Stefanie N. Bernas, Odette Leiter, Tara L. Walker and Gerd Kempermann, date: 11/05/2017, view: 370, Q&A: 0
    [Abstract] There are two neurogenic niches in the adult mammalian brain: the subventricular zone of the lateral ventricle and the subgranular zone of the hippocampal dentate gyrus. Cells from these areas can be isolated and maintained in vitro, using two different culture systems to assess their potential regarding proliferation and differentiation ...
    Sensitive Estimation of Flavor Preferences in STFP Using Cumulative Time Profiles
    Authors:  Aditya Singh and J. Balaji, date: 11/05/2017, view: 215, Q&A: 0
    [Abstract] Social transmission of food preference (STFP) is observed among rodents between a demonstrator and a naïve hungry observer. During social interaction, hungry observer receives information about safety of the food consumed by the demonstrator. This task has been implemented to develop a single trial non-aversive learning task in order to test ...
    Labeling Aversive Memory Trace in Mouse Using a Doxycycline-inducible Expression System
    Authors:  Jianyang Du and Erin E. Koffman, date: 10/20/2017, view: 370, Q&A: 0
    [Abstract] A memory trace, also known as a memory engram, is theorized to be a mechanism for physical memory storage in the brain (Silva et al., 2009; Josselyn, 2010) and memory trace is associated with a specific population of neurons (Liu et al., 2012; Ramirez et al., 2013). Labeling and stimulating those neurons will activate ...
    Isolation and Purification of Schwann Cells from Spinal Nerves of Neonatal Rat
    Authors:  Jinkun Wen, Dandan Tan, Lixia Li and Jiasong Guo, date: 10/20/2017, view: 443, Q&A: 0
    [Abstract] Primary cultured Schwann cells (SCs) are widely used in the investigation of the biology of SC and are important seed cells for neural tissue engineering. Here, we describe a novel protocol for harvesting primary cultured SCs from neonatal Sprague-Dawley (SD) rats. In the present protocol, dissociated SCs are isolated from the spinal nerves of ...
    Touchscreen-based Visual Discrimination and Reversal Tasks for Mice to Test Cognitive Flexibility
    Authors:  Karly M. Turner, Christopher G. Simpson and Thomas H. J. Burne, date: 10/20/2017, view: 309, Q&A: 0
    [Abstract] Reversal learning can be used to examine deficits in cognitive flexibility, which have been linked to a number of neuropsychiatric disorders including schizophrenia and addiction. However, methods of examining reversal learning have varied substantially between species. Touchscreen technology has allowed researchers to explore cognitive deficits ...
    Preparation of Teased Nerve Fibers from Rat Sciatic Nerve
    Authors:  Jinkun Wen, Lixia Li, Dandan Tan and Jiasong Guo, date: 10/05/2017, view: 428, Q&A: 0
    [Abstract] Compared to tissue sectioning techniques, the technique of teasing single nerve fibers provides a better way to understand the structures of myelin sheaths and axons of the peripheral myelinated nerves. This protocol describes a method for preparation of teased single nerve fibers from rat sciatic nerve. In this protocol, fixed nerves are teased ...
    Bioluminescence Monitoring of Neuronal Activity in Freely Moving Zebrafish Larvae
    [Abstract] The proof of concept for bioluminescence monitoring of neural activity in zebrafish with the genetically encoded calcium indicator GFP-aequorin has been previously described (Naumann et al., 2010) but challenges remain. First, bioluminescence signals originating from a single muscle fiber can constitute a major pitfall. Second, ...
    Stereotaxic Adeno-associated Virus Injection and Cannula Implantation in the Dorsal Raphe Nucleus of Mice
    Authors:  Patrícia A. Correia, Sara Matias and Zachary F. Mainen, date: 09/20/2017, view: 675, Q&A: 0
    [Abstract] Optogenetic methods are now widespread in neuroscience research. Here we present a detailed surgical procedure to inject adeno-associated viruses and implant optic fiber cannulas in the dorsal raphe nucleus (DRN) of living mice. Combined with transgenic mouse lines, this protocol allows specific targeting of serotonin-producing neurons in the ...
    Preparation of Primary Cultures of Embryonic Rat Hippocampal and Cerebrocortical Neurons
    [Abstract] This protocol aims at standardizing the procedure to obtain primary cultures of hippocampal and cerebrocortical neurons for in vitro experiments. Cultures should be prepared from cells isolated during embryonic development when neuronal precursor cells are not yet fully differentiated. This helps increasing the quality and quantity of ...