Abstract

In this webinar, we explore advancements in understanding DNA replication dynamics at the single-cell level, focusing on the innovative method of single-cell 5-ethynyl-2′-deoxyuridine sequencing (scEdU-seq). DNA replication, a vital process ensuring accurate genome duplication before cell division, varies across different epigenetic states and cell types. Using scEdU-seq, researchers can now measure replication speeds with high precision and observe how these speeds fluctuate during various stages of the S phase.

Join us as we delve into the temporal variations in DNA replication speed, the role of transcription-induced DNA damage in limiting replication speed, and the implications for developmental defects and tumorigenesis. The webinar will also highlight key genetic and pharmacological perturbations that influence DNA replication, offering insights into the complex interplay between replication dynamics and genomic integrity.

Highlights:

- Introduction to scEdU-seq: A novel method for measuring DNA replication speed in single cells.

- Insights into the variability of replication speed during the S phase of the cell cycle.

- The impact of transcription-induced DNA damage on replication dynamics.

- Discussion on the acceleration of replication in the late S phase.

- Applications of genetic and pharmacological perturbations to modulate replication speed.

- Implications for understanding developmental defects and cancer development.