Preparation of BS biofilms

Preparation of BS biofilms

The extracted BS was grown on solid LB plates. Seed colonies were first cultured in 4 ml of LB medium overnight at 37°C to obtain BS without biofilms. Then, 100μl of seed culture medium was resuspended and grown in 4 ml of MSgg medium overnight at 37°C to generate FCBS. Cell pellets collected from the FCBS suspension were resuspended in 1 ml of PBS, and 10μl of the resulted solution was spread on solid MSgg plates. Robust BS biofilms were produced after 2 days of culture at 30°C. Individually coated bacteria BCBS were prepared by homogenizing the films with PBS.

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