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DNA extraction
Last updated date: Mar 2, 2021 Views: 1245 Forks: 0
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Dear Kimberly,
Many thanks for getting in touch – raw DNA extraction yields from DNeasy PowerWater can be fairly low. In our case, most outputs ranged at ~900 ng total. You can obviously play with the kits descriptions a bit, e.g. extending beat beating, using higher input water volumes, etc. But even a few ng (and low 230 purity) would yield you plenty of DNA to amplify 16S from and sequence these with MinION!
All the best,
Max
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How to cite：
Readers should cite both the Bio-protocol preprint and the original research article where this protocol was used:
- Stammnitz, M(2021). DNA extraction. Bio-protocol Preprint. bio-protocol.org/prep893.
- Urban, L., Holzer, A., Baronas, J. J., Hall, M. B., Braeuninger-Weimer, P., Scherm, M. J., Kunz, D. J., Perera, S. N., Martin-Herranz, D. E., Tipper, E. T., Salter, S. J. and Stammnitz, M. R.(2021). Freshwater monitoring by nanopore sequencing. eLife. DOI: 10.7554/eLife.61504
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