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Cryo-EM structural analysis of Ad5-3M
Last updated date: Jan 27, 2021 Views: 925 Forks: 0
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We followed established protocols in cryo-EM single particle image processing. There are online tutorials available for the cryo-EM software packages we used:
EMAN2 https://blake.bcm.edu/emanwiki/EMAN2
Relion 2.0 https://www3.mrc-lmb.cam.ac.uk/relion/index.php?title=Main_Page
cisTEM https://cistem.org/
In addition, the Simons Electron Microscopy Center at NYSBC has several YouTube videos that describe the Cryo-EM single-particle analysis method:
SEMC 2017 Cryo-EM Course - Single-particle analysis (part I: Intro and types of samples) https://www.youtube.com/watch?v=736YopwAEZ4
SEMC 2017 Cryo-EM Course - Single Particle Analysis (Part II: Data Analysis and Reconstruction) https://www.youtube.com/watch?v=UDcN3iWOlsw
SEMC 2019 Cryo-EM Course - Single-particle analysis (part II: Data Analysis & workflow - Reza Khayat: https://www.youtube.com/watch?v=VdPT9B-q_lc
Also, Grant Jenson, a professor at Caltech, has an online course on getting started in cryo-EM:
Grant Jensen “Getting started in cryo-EM” http://cryo-em-course.caltech.edu/
- Stewart, P(2021). Cryo-EM structural analysis of Ad5-3M. Bio-protocol Preprint. bio-protocol.org/prep785.
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