Cryo-EM structural analysis of Ad5-3M

Phoebe L. Stewart

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Cryo-EM structural analysis of Ad5-3M

PS Phoebe L. Stewart

Last updated date: Jan 27, 2021 Views: 940 Forks: 0

An abbreviated version of this protocol was published in Science Translational Medicine in Nov, 2020

Systemic cancer therapy with engineered adenovirus that evades innate immunity

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We followed established protocols in cryo-EM single particle image processing. There are online tutorials available for the cryo-EM software packages we used:

EMAN2 https://blake.bcm.edu/emanwiki/EMAN2

Relion 2.0 https://www3.mrc-lmb.cam.ac.uk/relion/index.php?title=Main_Page

cisTEM https://cistem.org/

In addition, the Simons Electron Microscopy Center at NYSBC has several YouTube videos that describe the Cryo-EM single-particle analysis method:

SEMC 2017 Cryo-EM Course - Single-particle analysis (part I: Intro and types of samples) https://www.youtube.com/watch?v=736YopwAEZ4

SEMC 2017 Cryo-EM Course - Single Particle Analysis (Part II: Data Analysis and Reconstruction) https://www.youtube.com/watch?v=UDcN3iWOlsw

SEMC 2019 Cryo-EM Course - Single-particle analysis (part II: Data Analysis & workflow - Reza Khayat: https://www.youtube.com/watch?v=VdPT9B-q_lc

Also, Grant Jenson, a professor at Caltech, has an online course on getting started in cryo-EM:

Grant Jensen “Getting started in cryo-EM” http://cryo-em-course.caltech.edu/

How to cite：

Readers should cite both the Bio-protocol preprint and the original research article where this protocol was used:

Stewart, P(2021). Cryo-EM structural analysis of Ad5-3M. Bio-protocol Preprint. bio-protocol.org/prep785.
Atasheva, S., Emerson, C. C., Yao, J., Young, C., Stewart, P. L. and Shayakhmetov, D. M.(2020). Systemic cancer therapy with engineered adenovirus that evades innate immunity . Science Translational Medicine 12(571). DOI: 10.1126/scitranslmed.abc6659