Creating acetic acid-induced artifical gastric ulcers in rats

 Sprague Dawley (SD) rats weighing 200 to 250 g (female) were used. After a 24 h fast, rats were anaesthetized by an intraperitoneal injection of ketamine (80 mg/kg body weight). The stomach was then isolated. A hollow O-ring mold (OD=10 mm) with an inlet and outlet was placed in the gastric body through a small incision at the fundus region. The mold was gripped tightly by the surgeon’s fingers, and 75% acetic acid was injected into the mould through the inlet. After 90 seconds, two symmetric gastric ulcers were created on the anterior and posterior walls of the stomach. Next, DPBS (Dulbecco’s phosphate-buffered saline, Sigma-Aldrich) was injected to wash away the excess acid. After the ulcers were created, two symmetric gastric ulcers in one stomach were created.

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