Pre-processing of fiber photometry signal

Full data, code, and details for each script can be found at https://doi.org/10.5061/dryad.3xsj3txcf. Attached are modified versions of the pre-processing scripts that will more easily allow someone to pre-process their data using the least mean squares regression method. 

To pre-process a raw doric FP file, open the script Basic_FP_Processing.m and change the directory to match the folder where your raw doric files are. Running the script will call calculateDF_F0.m and subtractReferenceAndSave.m, resulting in a .csv with the prefix "PROCESSED_" for each file processed. Inside this output file, there will be 4 columns: Time, Reference (DF/F0), Signal (DF/F0), and Corrected. Corrected is the difference between column 2 and 3. We analyzed the reference and signal channels separately instead of using the reference subtracted column. 

If not using a Doric system, the required format of the data is a .csv with 3 columns: timestamps, reference channel, and signal channel. The z-scoring mentioned in the methods was done in the next step of the pipeline, and the function nanzscore was used (uploaded here). 

Category