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Preprint

Peripheral nervous system (PNS) axotomy assay

SS Sean D Speese
ML Mary A Logan

Last updated date: Nov 10, 2020 Views: 1130 Forks: 0

original An abbreviated version of this protocol was published in eLife in Aug, 2017
A novel Drosophila injury model reveals severed axons are cleared through a Draper/MMP-1 signaling cascade
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Hi Lorenzo,

   We recently published our entire workflow in a methods chapter that includes numerous videos.  Here is the link to this paper:


https://link.springer.com/protocol/10.1007%2F978-1-0716-0585-1_24


Hopefully you will be able to download the paper and watch the videos.  If for some reason you do not have access I have uploaded the PDF of the book.  Our methods are in chapter 24.  Please contact us if you don't have access to this publication on-line and we can get the videos to you.



Sean Speese

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pdf 2020_Book_AxonDegeneration.pdf download
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How to cite：
Readers should cite both the Bio-protocol preprint and the original research article where this protocol was used:
  1. Speese, S and Logan, M(2020). Peripheral nervous system (PNS) axotomy assay. Bio-protocol Preprint. bio-protocol.org/prep618.
  2. Purice, M. D., Ray, A., Münzel, E. J., Pope, B. J., Park, D. J., Speese, S. D. and Logan, M. A.(2017). A novel Drosophila injury model reveals severed axons are cleared through a Draper/MMP-1 signaling cascade. eLife. DOI: 10.7554/eLife.23611

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