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Chemicals and synthetic DNA

MR Monica Rosas-Lemus

Last updated date: Oct 12, 2020 Views: 801 Forks: 0

original An abbreviated version of this protocol was published in Science Signaling in Sep, 2020
High-resolution structures of the SARS-CoV-2 2′-O-methyltransferase reveal strategies for structure-based inhibitor design
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Hello Janina,

 

The manual edits are just a second inspection after codon optimization (Gensmart) to identify and correct possible artificial ribosome binding sites produced by the software. You can request our plasmids from BEI. Also, please find bellow the optimized sequences used for this study. 

 

Hope this is helpful.

 

All the best,

Monica

 

 

NSP10 sequence+ Start and Stop codons

ATGGCGGGAAACGCAACTGAGGTACCCGCTAATTCGACCGTGCTGAGCTTCTGCGCGTTTGCAGTTGATGCGGCTAAAGCGTATAAGGACTACTTGGCTTCCGGTGGTCAGCCGATCACGAACTGCGTTAAAATGTTGTGCACGCACACCGGCACCGGCCAGGCAATTACCGTTACCCCGGAGGCGAACATGGATCAAGAGAGCTTTGGTGGCGCGAGCTGCTGCCTGTACTGCCGCTGCCATATTGACCACCCGAATCCAAAAGGCTTCTGCGACCTGAAGGGCAAA

TACGTGCAAATCCCGACCACCTGTGCCAATGATCCGGTTGGTTTCACCCTGAAGAACACCGTGTGCACTGTCTGTGGTATG TGG AAA GGC TATGG

TTGTTCTTGTGACCAACTGCGTGAACCGATGTTACAGTAA


 

 

Nsp16 sequence+ Start and Stop codons

ATGTCAAGTCAGGCATGGCAACCCGGAGTAGCTATGCCGAATCTGTACAAGATGCAGCGTATGCTGTTGGAAAAATGTGATCTGCAAAACTACGGCGA

CTCTGCTACTCTCCCAAAGGGCATTATGATGAACGTCGCCAAATACACCCAGCTTTGCCAATATCTGAACACCTTGACCCTCGCAGTGCCGTACA

ACATGCGTGTTATCCACTTTGGTGCGGGTAGCGACAAAGGCGTTGCTCCGGGTACGGCTGTGTTGCGCCAGTGGCTGCCGACCGGTACATTGCTG

GTGGATTCTGACCTGAATGATTTTGTCAGCGACGCAGATTCCACCTTAATCGGCGACTGTGCGACCGTTCACACCGCGAATAAATGGGATTTGAT

TATCAGCGACATGTATGATCCGAAGACCAAGAACGTGACGAAAGAGAACGACTCC AAA GAG GGCTTCTTCACCTATATCTGCGGTTTTATCCAGC

AGAAACTGGCACTGGGCGGCTCCGTGGCGATTAAAATCACCGAACATTCTTGGAATGCGGATCTGTACAAACTTATGGGTCATTTCGCATGGTGG

ACCGCGTTCGTTACCAATGTGAACGCCAGCAGCTCAGAAGCTTTCCTGATCGGGTGCAATTACCTGGGTAAACCGCGTGAGCAGATCGATGGTTA

TGTTATGCACGCGAACTACATTTTTTGGCGTAATACGAACCCGATTCAACTGTCGAGCTATAGCTTGTTTGATATGAGCAAGTTCCCGCTGAAGC

TGCGTGGTACTGCGGTAATGTCC CTT AAA GAG GGC CAAATTAACGACATGATTCTGAGCTTGCTGAGTAAGGGCCGTCTGATCATCCGCGAAAAC

AACCGCGTTGTTATTAGCTCGGACGTCCTGGTTAATAACTAA

 



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How to cite：
Readers should cite both the Bio-protocol preprint and the original research article where this protocol was used:
  1. Rosas-Lemus, M(2020). Chemicals and synthetic DNA. Bio-protocol Preprint. bio-protocol.org/prep541.
  2. Rosas-Lemus, M., Minasov, G., Shuvalova, L., Inniss, N. L., Kiryukhina, O., Brunzelle, J. and Satchell, K. J. F.(2020). High-resolution structures of the SARS-CoV-2 2′-O-methyltransferase reveal strategies for structure-based inhibitor design . Science Signaling 13(651). DOI: 10.1126/scisignal.abe1202

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