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Plasmids generation

HB Harihar Basnet

Last updated date: Nov 24, 2019 Views: 1116 Forks: 0

original An abbreviated version of this protocol was published in eLife in Mar, 2019
Flura-seq identifies organ-specific metabolic adaptations during early metastatic colonization
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Primers used for cloning the constructs described in the manuscript are described in Supplementary file 6. CD (Addgene 35102), and UPRT (Addgene 47110) were used as template for PCR for subcloning. RFP and IRES were amplified using pTRIPZ (Dharmacon) as template. The PCR products were either ligated using DNA Ligase after restriction enzyme digestion and/or by Gibson Assembly.

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How to cite：
Readers should cite both the Bio-protocol preprint and the original research article where this protocol was used:
  1. Basnet, H(2019). Plasmids generation. Bio-protocol Preprint. bio-protocol.org/prep47.
  2. Basnet, H., Tian, L., Ganesh, K., Huang, Y., Macalinao, D. G., Brogi, E., Finley, L. W. and Massagué, J.(2019). Flura-seq identifies organ-specific metabolic adaptations during early metastatic colonization. eLife. DOI: 10.7554/eLife.43627

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