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Preprint

Immunostaining

JY Junhua Yang

Last updated date: Aug 24, 2020 Views: 925 Forks: 0

original An abbreviated version of this protocol was published in eLife in Apr, 2016
Astrocytes contribute to synapse elimination via type 2 inositol 1,4,5-trisphosphate receptor-dependent release of ATP
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For the IP3R2 immunostaining, we used a standard protocol which consists of perfusion, fixation, permeabilization, and antibody incubation. After the perfusion, the fixation is important. We found that 6-8 hours in 4% PFA is enough. Antigen retrieval could help at some time, especially for the old sample.

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How to cite：
Readers should cite both the Bio-protocol preprint and the original research article where this protocol was used:
  1. Yang, J(2020). Immunostaining. Bio-protocol Preprint. bio-protocol.org/prep463.
  2. Yang, J., Yang, H., Liu, Y., Li, X., Qin, L., Lou, H., Duan, S. and Wang, H.(2016). Astrocytes contribute to synapse elimination via type 2 inositol 1,4,5-trisphosphate receptor-dependent release of ATP. eLife. DOI: 10.7554/eLife.15043

Category

Immunology

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