1. Prepare 300ml of a 1% agar solution (3g), heat, and dissolve agar in the microwave.

2. Grind 1.0 kg of food pellets and place into the mixer.

3. Add the appropriate amount of drug.

4. Encapsulated rapamycin was purchased directly from Rapamycin Holdings, Inc (now Emtora Biosciences^TM), which also comes with Eudragit (encapsulation material used in eRAPA^TM). For the amount of encapsulated rapamycin, multiply the amount of active rapamycin needed by 100 and divide by the “percentage active” written on the bottle.

Example: 14mg/kg of active rapamycin in diet. If “10% active” on the bottle, multiply 14mg/kg by 100 and divide by 10 (140mg) for the amount needed for 1 kg of food. Add the same weight of Eudragit in another 1kg of food to make the control food.

5. Let agar cool to handling temperature.

6. Add hot agar to the powdered food/drug, pouring slowly.

7. Increase the speed of mixing

8. Add 200 ml of water

9. Mix for another 2 minutes

10. Stop mixer

11. Prepare food pellets

12. package in saran wrap and aluminum foil, label each package, vacuum seal.

13. Store at -20 C

14. Check animals daily, and once per week check food levels and fill to top.