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Zebrafish strains, husbandry, embryo treatments, and morpholinos

JV Julien Vermot

Last updated date: Aug 5, 2020 Views: 973 Forks: 0

original An abbreviated version of this protocol was published in eLife in Sep, 2019
Mechanically activated piezo channels modulate outflow tract valve development through the Yap1 and Klf2-Notch signaling axis
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We have not assessed the activity of this promoter in the pancreas. We would be happy to send the construct over to you. I attached the promoter sequence and primers for amplification of the 3.2 kb promoter sequence we used.

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word Promoteur PIEZO 1- 3,2 kb.docx download
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How to cite：
Readers should cite both the Bio-protocol preprint and the original research article where this protocol was used:
  1. Vermot, J(2020). Zebrafish strains, husbandry, embryo treatments, and morpholinos. Bio-protocol Preprint. bio-protocol.org/prep435.
  2. Duchemin, A., Vignes, H. and Vermot, J.(2019). Mechanically activated piezo channels modulate outflow tract valve development through the Yap1 and Klf2-Notch signaling axis. eLife. DOI: 10.7554/eLife.44706

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