M13 phage for activating immune cells of DCs and M1 macrophages

BMDCs were seeded in 6-well plate with a density of 5×10⁵ per well and incubated with M13 phage (1×10¹⁰ PFU) for 24 h. Then, flow cytometry analysis of mature DCs stained surface marker with CD11c, CD80 and CD86. 

For M1 phenotype macrophages polarization, RAW 264.7 cells were seeded in 6-well plate (1×10⁴ per well) and 20 ng/mL IL-4 was added to induce differentiation of M2 macrophages for 24 h. M13 phage (1×10¹⁰ PFU) were co-cultured with M2 macrophages for another 24 h, Subsequently. flow cytometry analysis of M1 phenotype highly expressed CD86. 

Category