Preparation of AGP

1.    PBS buffer solution (PH 7.4) was used for washing Synechococcus elongatus PCC7942 by centrifugation (3000 r, 5 min)

2.    Resuspended PCC7942 in PBS with 500 μM Na₂CO₃

3.    Prepared 8% sodium alginate with deionized water

4.    Mix 8% sodium alginate with PCC7942 solution by equal volume

5.    Prepared 3% calcium chloride with deionized water in a beaker with a magnetically stirred rotor

6.    Alginate- PCC7942 solution was injected into calcium chloride by 1ml syringe on the magnetic stirrer

7.    Alginate- PCC7942 gel balls were washed with PBS buffer solution three times

8.    The inner lining of AGP to attach wounds is 0.22-um porous polytetrafluoroethylene membrane

9.    Put alginate- PCC7942 gel balls on the polytetrafluoroethylene membrane. The airtight polyurethane film is used as the backing layer.

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