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Preprint

Genotyping CCR7-flox mice

MB Milena Bogunovic

Last updated date: Oct 27, 2025 Views: 11 Forks: 0

original An abbreviated version of this protocol was published in Science Immunology
Gut-resident CX3CR1hi macrophages induce tertiary lymphoid structures and IgA response in situ
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Ccr7-flox mice were donated to Jax website.

Genenral information: https://www.jax.org/strain/036464

Genotyping: https://www.jax.org/Protocol?stockNumber=036464&protocolID=41067

The construct structure was included in the orginal manuscript (PMID: 32276965)

Any other questions should be directed to Iannis Aifantis, NYU School of Medicine, whose lab developed these mice.

 

Milena Bogunovic

Copyright: © 2025 The Author(s); This is an open-access article under the CC BY-NC license (https://creativecommons.org/licenses/by-nc/4.0/).
How to cite：
Readers should cite both the Bio-protocol preprint and the original research article where this protocol was used:
  1. Bogunovic, M(2025). Genotyping CCR7-flox mice. Bio-protocol Preprint. bio-protocol.org/prep2869.
  2. Koscsó, B., Kurapati, S., Rodrigues, R. R., Nedjic, J., Gowda, K., Shin, C., Soni, C., Ashraf, A. Z., Purushothaman, I., Palisoc, M., Xu, S., Sun, H., Chodisetti, S. B., Lin, E., Mack, M., Kawasawa, Y. I., He, P., Rahman, Z. S. M., Aifantis, I., Shulzhenko, N., Morgun, A. and Bogunovic, M.(2020). Gut-resident CX3CR1hi macrophages induce tertiary lymphoid structures and IgA response in situ . Science Immunology 5(46). DOI: 10.1126/sciimmunol.aax0062

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