Sulfate detection by ion-exchange chromatography

https://doi.org/10.1038/s41598-021-98588-3

Sulfate detection by ion‑exchange chromatography. The sulfate content of fucoidans substrates as
well as sulfate release after sulfatase treatment can be analyzed by high performance anion exchange chromatography
(HPAEC) using a Dionex ICS-5000 chromatography system equipped with a GP40 gradient pump and an
ED40 electrochemical detector. The ions are separated on an HC-AS11 anion-exchange column (4 × 250 mm;
Dionex) with accompanying HC-AG11 guard column (4 × 50 mm; Dionex). Elution was performed with 35 mM
NaOH using an isocratic flow rate of 1.5 ml min−1. 

Background signal and noise originating from the eluent was reduced using an anion self-regenerating suppressor (AERS-500, Dionex) with a current of 180 mA (Tip from the author of the paper: This suppression is important for good results). 

Sulfate concentration is then deduced from the signal intensity and calculated from a standard sulfate calibration curve
using known concentrations of Na2SO4.
Best regards

Hilsen/Best regards   Anne S. Meyer Professor, Head of Section Protein Chemistry & Enzyme Technology  Group leader, Enzyme Technology DTU Bioengineering, Department of Biotechnology & Biomedicine Technical University of Denmark DTU Building 221 2800 Kgs. Lyngby, Denmark asme@dtu.dk http://www.bioengineering.dtu.dk/english/researchny/research-sections/section-for-protein-chemistry-and-enzyme-technology