4.8.1. BSA Denaturation InhibitionAssay

BSA Denaturation Inhibition Assay

Inhibition of albumin denaturation was performed by following a standard method (Jyothilakshmi et al., 2020) with slight modification. Different concentrations of samples were mixed with 1.8 mL of 1% BSA solution. The BSA solution were fully solubilized by stirring at 400 rpm for 20 min by adjusting the pH to 6.5. The BSA solution were fully solubilized by stirring at 400 rpm for 20 min. The reaction mixture was carried out at 37 °C for 20 min and then heated to 57 °C for10 min. After cooling, turbidity was generated and measured the absorbance at 660 nm wavelength using a UV−vis spectrophotometer. Diclofenac sodium and a solution without a sample were considered as a standard and control respectively. The experiments were performed in triplicates. The relative percentage inhibition of protein denaturation was calculated. %Inhibition = [(Abs control- Abs sample) /Abs control] × 100