2.4. Incubation in Simulated Body Fluid (SBF)

Simulated body fluid (SBF) (500 ml) was prepared as described by Jalota et al [1]. The chemicals used to prepare the SBF and the amounts added are listed in Table 1. A beaker of deionized water was placed on a magnetic stirrer at 37°C. Each chemical was weighed using an electronic balance and added to the deionized water in the order shown in Table 1. An electronic pH meter (Mettler Toledo, EL20, Columbus, OH, USA) was then used to measure the exact pH of the solution, and hydrochloric acid was slowly added until the solution reached a pH of 7.4. The beaker was then covered with aluminum foil and left on the magnetic stirrer overnight. The next day, the solution was filtered through a 0.2 µm (pore size) filter and sterile sealed (ready for use). 

Table 1: Composition of SBF (according to Jalota et al. [1])

1.            Jalota, S.; Bhaduri, S.B.; Tas, A.C. Using a synthetic body fluid (sbf) solution of 27 mm hco3− to make bone substitutes more osteointegrative. Materials Science and Engineering: C 2008, 28, 129-140.http://dx.doi.org/10.1016/j.msec.2007.10.058